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  4. Dog Interleukin 1β (IL- 1β) ELISA Kit

Dog Interleukin 1β (IL- 1β) ELISA Kit

  • 中文名稱：
    犬白介素1β(IL-1β)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13836c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬白介素1β(IL-1β)酶聯(lián)免疫試劑盒（CSB-E13836c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL1B含量。IL1B，即白介素-1β，是一種強(qiáng)效的促炎細(xì)胞因子，主要由單核細(xì)胞、巨噬細(xì)胞和樹(shù)突狀細(xì)胞產(chǎn)生。它在炎癥和免疫反應(yīng)中起關(guān)鍵作用，通過(guò)激活I(lǐng)L-1受體和下游信號(hào)通路，調(diào)節(jié)細(xì)胞增殖、分化和凋亡。IL1B的研究對(duì)于理解炎癥性疾病的發(fā)生機(jī)制和開(kāi)發(fā)治療策略具有重要意義。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，該產(chǎn)品適用于科研領(lǐng)域，如探究犬類模型中的炎癥性疾病病理過(guò)程、評(píng)估免疫調(diào)節(jié)劑或抗炎藥物的干預(yù)效果，以及動(dòng)物疫苗研發(fā)中的免疫應(yīng)答研究，為獸醫(yī)學(xué)和比較醫(yī)學(xué)領(lǐng)域的實(shí)驗(yàn)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %83-100
    1:2Average %94
    Range %91-99
    1:4Average %95
    Range %90-105
    1:8Average %94
    Range %86-100
  • 回收率：
    The recovery of dog IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-102
    EDTA plasma (n=4)9790-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.585 2.675 2.630 2.512
    2502.319 2.412 2.366 2.248
    1251.957 1.972 1.965 1.847
    62.51.432 1.453 1.443 1.325
    31.250.744 0.719 0.732 0.614
    15.630.517 0.508 0.513 0.395
    7.80.385 0.377 0.381 0.263
    00.118 0.117 0.118 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 亞細(xì)胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫(kù)鏈接：