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  4. Mouse Eotaxin 2 ELISA Kit

Mouse Eotaxin 2 ELISA Kit

  • 中文名稱：
    小鼠嗜酸粒細(xì)胞趨化蛋白Eotaxin 2(Eotaxin 2/CCL24)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07278m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠嗜酸粒細(xì)胞趨化蛋白Eotaxin 2(Eotaxin 2/CCL24)酶聯(lián)免疫試劑盒（CSB-E07278m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CCL24含量。CCL24，又稱嗜酸性粒細(xì)胞趨化蛋白2（eotaxin-2）和骨髓祖細(xì)胞抑制因子2（MPIF-2），是一種CC趨化因子，位于人類基因組第7號(hào)染色體。CCL24通過與其唯一受體CCR3結(jié)合，主要介導(dǎo)特應(yīng)性疾病、寄生蟲感染和全身性疾病，同時(shí)促進(jìn)細(xì)胞運(yùn)輸并調(diào)節(jié)炎癥和纖維化活動(dòng)。研究顯示，CCL24在纖維化疾病中發(fā)揮重要作用，其表達(dá)水平與多種纖維炎癥性疾病相關(guān)。試劑盒檢測(cè)范圍為15.6 pg/ml- 1000 pg/ml，靈敏度為3.9 pg/ml。為探索炎癥相關(guān)疾病的分子機(jī)制、評(píng)估藥物干預(yù)效果或構(gòu)建疾病模型提供可靠工具；適用于體外培養(yǎng)細(xì)胞上清、動(dòng)物模型體液及組織樣本的CCL24表達(dá)水平分析，滿足科研人員對(duì)趨化因子網(wǎng)絡(luò)調(diào)控研究的多元化需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccl24 ELISA Kit; Scya24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; Small-inducible cytokine A24 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/ml - 1000 pg/ml
  • 靈敏度：
    3.9 pg/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    80-96

    1:2

    Average %

    94

    Range %

    91-105

    1:4

    Average %

    96

    Range %

    92-99

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of mouse eotaxin 2 /CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    97

    94-101

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.612

    2.634

    2.623

    2.507

    500

    2.200

    2.267

    2.233

    2.117

    250

    1.453

    1.528

    1.491

    1.374

    125

    0.753

    0.729

    0.741

    0.625

    62.5

    0.349

    0.329

    0.339

    0.222

    31.2

    0.242

    0.253

    0.248

    0.131

    15.6

    0.180

    0.184

    0.182

    0.066

    0

    0.113

    0.120

    0.116

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
  • 基因功能參考文獻(xiàn)：
    1. the increased airway eosinophils and mucous cell metaplasia in house dust mite-challenged Cd163-/- mice are mediated by augmented CCL24 production PMID: 26376364
    2. this study shows that TPL-2 inhibits Ccl24 expression in lung dendritic cells, and blockade of Ccl24 prevents the exaggerated airway eosinophilia and lung inflammation in mice given house dust mites-pulsed Map3k8-/- dendritic cells PMID: 27484038
    3. production in M-CSF-induced bone marrow-derived macrophages is synergistically induced by IL-4 and IL-10 PMID: 25267883
    4. Autologous transfer of peritoneal macrophages in to the airways of asthmatic mice reduces eotaxin production. PMID: 24077949
    5. Data indicate that eotaxin 2 (CCL-24), which is known to influence eosinophil migration, was highly up-regulated in broncho-alveolar macrophages (BAMs). PMID: 23685352
    6. The oesophageal production of CCL24 upon IL-13 stimulation is sufficient to promote eosinophil migration. PMID: 20030665
    7. Results demonstrate a cooperative mechanism between interleukin-13 and eotaxin-2, where IL-13 mediates allergen-induced eotaxin-2 expression, and eotaxin-2 mediates IL-13-induced airway eosinophilia. PMID: 15647285
    8. Distinct acidic and basic residues within CCR3 determine both receptor expression and activation by the eotaxins. PMID: 16102831
    9. The eotaxin-2 pathway plays a fundamental role in eosinophil recruitment during ovalbumin-induced experimental asthma. PMID: 16210640
    10. eosinophils via chemokine (C-C) receptor 3 have a central role in chronic allergic airway disease PMID: 17060636
    11. CCL24 seems important for recruitment of eosinophils into BAL. PMID: 18850374

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis.
  • 數(shù)據(jù)庫鏈接：