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  4. Rat Interleukin 21,IL-21 ELISA Kit

Rat Interleukin 21,IL-21 ELISA Kit

  • 中文名稱：
    大鼠白介素21(IL-21)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11843r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素21(IL-21)酶聯(lián)免疫試劑盒（CSB-E11843r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL21含量。IL21，即白細(xì)胞介素-21，是一種由CD4+T細(xì)胞和自然殺傷T(NKT)細(xì)胞產(chǎn)生的細(xì)胞因子，屬于IL-15/IL-21家族。它通過(guò)結(jié)合IL-21R受體激活JAK1和JAK3信號(hào)，進(jìn)而調(diào)節(jié)T、B、NK細(xì)胞和DC的增殖與分化，在免疫調(diào)節(jié)中發(fā)揮重要作用。IL21在多種免疫疾病中扮演關(guān)鍵角色，如自身免疫性疾病和癌癥。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于體外定量分析大鼠外周血或組織樣本中IL-21的動(dòng)態(tài)變化，可廣泛應(yīng)用于免疫調(diào)節(jié)機(jī)制研究、炎癥性疾病動(dòng)物模型評(píng)估或藥物干預(yù)后免疫指標(biāo)變化的科研分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Il21Interleukin-21 ELISA Kit; IL-21 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %85-96
    1:2Average %89
    Range %84-95
    1:4Average %103
    Range %99-108
    1:8Average %99
    Range %94-105
  • 回收率：
    The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-101
    EDTA plasma (n=4)9288-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.601 2.544 2.573 2.456
    5001.921 1.952 1.937 1.820
    2501.198 1.122 1.160 1.043
    1250.735 0.709 0.722 0.605
    62.50.425 0.459 0.442 0.325
    31.20.272 0.257 0.265 0.148
    15.60.181 0.172 0.177 0.060
    00.116 0.118 0.117
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-15/IL-21 family
  • 數(shù)據(jù)庫(kù)鏈接：