Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)酶聯(lián)免疫試劑盒（CSB-E08729r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SDF1a/CXCL12含量。SDF1a/CXCL12是重要的趨化因子。其背景上，在胚胎發(fā)育、免疫調(diào)節(jié)等生理過程發(fā)揮關(guān)鍵作用。研究機(jī)制方面，與受體CXCR4結(jié)合，介導(dǎo)細(xì)胞遷移、增殖和存活，在腫瘤轉(zhuǎn)移、炎癥等病理進(jìn)程中有重要影響，是相關(guān)疾病治療潛在靶點(diǎn)。試劑盒檢測(cè)范圍為0.78 pg/mL-50 pg/mL，可廣泛應(yīng)用于神經(jīng)再生、造血調(diào)控、免疫細(xì)胞趨化性等基礎(chǔ)研究領(lǐng)域，為探索SDF - 1a/CXCL12在疾病模型中的動(dòng)態(tài)變化提供高特異性檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

SDF1a/CXCL12
Uniprot No.：

Q9QZD1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

0.78 pg/mL-50 pg/mL
靈敏度：

0.195 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	83-97
1:200	Average %	97
	Range %	91-103
1:400	Average %	84
	Range %	80-88
1:800	Average %	89
	Range %	81-95

回收率：

The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-96
EDTA plasma (n=4)	97	94-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.