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  4. Rat cluster of differentiation 4,CD4 ELISA Kit

Rat cluster of differentiation 4,CD4 ELISA Kit

  • 中文名稱：
    大鼠CD4分子(CD4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12928r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠CD4分子(CD4)酶聯(lián)免疫試劑盒（CSB-E12928r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD4含量。CD4是人體免疫系統(tǒng)的關鍵靶點。它是T細胞表面糖蛋白，輔助免疫細胞識別外來抗原。在研究機制上，CD4可與抗原呈遞細胞結合，激活T細胞，啟動免疫應答，在抗感染、抗腫瘤等方面作用重大，也是艾滋病病毒主要攻擊對象。試劑盒檢測范圍為0.78 U/mL-50 U/mL，適用于免疫學基礎研究、自身免疫性疾病模型評估、感染性疾病機制探索等方向，可為實驗動物模型中T細胞功能分析、免疫調節(jié)藥物開發(fā)等研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    Cd4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD antigen CD4
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.78 U/mL-50 U/mL
  • 靈敏度：
    0.195 U/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 97    
    Range % 87-102    
    1:2 Average % 101    
    Range % 94-105    
    1:4 Average % 99    
    Range % 88-103    
    1:8 Average % 105    
    Range % 98-109    
  • 回收率：
    The recovery of rat CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 85-98    
    EDTA plasma (n=4) 98 89-104    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    U/ml OD1 OD2 Average Corrected    
    50 2.656 2.587 2.622 2.508    
    25 2.171 2.062 2.117 2.003    
    12.5 1.438 1.388 1.413 1.299    
    6.25 0.720 0.715 0.718 0.604    
    3.12 0.412 0.424 0.418 0.304    
    1.56 0.261 0.252 0.257 0.143    
    0.78 0.176 0.183 0.180 0.066    
    0 0.112 0.115 0.114 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
  • 基因功能參考文獻：
    1. Adoptive transfusion of CD4+CD25+ Regulatory T Cells into Experimental Autoimmune Neuritis model rats has significant therapeutic effects. PMID: 29772575
    2. Increased C4d deposition may be amenable to identify early myocardial infarction after cardiac arrest. PMID: 26467126
    3. Data show several homologies across human, rat and mouse in the kinetic of loss of several LAgs such as CD5, CD4 and CD28. PMID: 21745657
    4. testicular macrophages expressed the markers CD45 and MAC387 and most also expressed CD4. PMID: 11994538
    5. C4d deposition and cellular infiltrates as markers of acute rejection in rat models of orthotopic lung transplantation. PMID: 18622289
    6. In this EAE model, perivascular cells, microglia and macrophages showed different dynamics; microglial cells expressed CD4 during the recovery phase, suggesting microglial CD4 expression in the resolution of the immune response. PMID: 19246105
    7. Clear trend toward worse graft survival in patients with focal C4d after kidney transplantaation. PMID: 19344468
    8. Capillary C4d deposition is a marker of antibody-mediated rejection in kidney transplantation and can be detected by various staining methods. PMID: 19376373

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  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 數(shù)據(jù)庫鏈接：