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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Pig interleukin 10,IL-10 ELISA Kit
  • 中文名稱：
    豬白介素10(IL-10)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06779p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素10(IL-10)酶聯(lián)免疫試劑盒（CSB-E06779p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL10含量。IL10是一種重要的細(xì)胞因子。其背景在于具有免疫調(diào)節(jié)功能，可抑制炎癥反應(yīng)。研究機(jī)制方面，它主要通過(guò)與受體結(jié)合，激活細(xì)胞內(nèi)信號(hào)通路，調(diào)節(jié)免疫細(xì)胞的活性和功能，減少促炎因子產(chǎn)生，在免疫相關(guān)疾病研究中備受關(guān)注。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于動(dòng)物免疫機(jī)制研究、疫苗效力評(píng)估或炎癥性疾病模型的生物標(biāo)志物分析。該產(chǎn)品為獸醫(yī)病理學(xué)、比較醫(yī)學(xué)及畜牧養(yǎng)殖領(lǐng)域的免疫調(diào)控研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %81-103
    1:2Average %95
    Range %90-105
    1:4Average %95
    Range %90-108
    1:8Average %96
    Range %82-112
  • 回收率：
    The recovery of pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9585-109
    EDTA plasma (n=4)9792-106
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.178 2.152 2.165 2.015
    2001.584 1.560 1.572 1.422
    1000.981 0.950 0.966 0.816
    500.568 0.564 0.566 0.416
    250.372 0.361 0.367 0.217
    12.50.296 0.302 0.299 0.149
    6.250.252 0.248 0.250 0.100
    00.152 0.148 0.150 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型：細(xì)胞培養(yǎng)上清液

樣品信息：其他

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)： 結(jié)果很滿意，也是對(duì)比了別家的試劑盒，最后發(fā)現(xiàn)還是華美的好用，我用的是細(xì)胞培養(yǎng)基上清，別家的試劑盒有干擾因素，華美的沒(méi)有，試了好幾家，也就這個(gè)牌子的好用。

By 高老師

樣品類型：血清

樣品信息：其他 豬

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)： 標(biāo)曲很好，結(jié)果在標(biāo)曲范圍之內(nèi)，符合預(yù)期結(jié)果。

By 謝老師

靶點(diǎn)詳情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 基因功能參考文獻(xiàn)：
    1. The PRRSV vaccination induced higher levels of IL-10 expression in the first week and this may be why the CSFV vaccination is immunosuppressed. PMID: 27938685
    2. These data indicate that the type 2 porcine reproductive and respiratory syndrome virus N protein plays an important role in IL-10 induction and the N-N non-covalent domain is associated with this activity. PMID: 25614594
    3. The effects of semen, spermatozoa in extender, or extender alone on the expression of TGF-beta1, IL-10, and IL-6 in ovarian follicles are reported. PMID: 24020676
    4. Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-gamma+CD4+ regulatory T cells to control transplant arteriosclerosis. PMID: 23359497
    5. Porcine reproductive and respiratory syndrome virus strain CH-1a could significantly up-regulate IL-10 production through p38 MAPK activation. PMID: 22909062
    6. Boar seminal plasma contained TGF- beta1 and IL-10 but with high individual variation. PMID: 22482797
    7. Changes in interleukin-10 mRNA expression are predictive for 9-day survival of pigs in an emergency preservation and resuscitation model. PMID: 20163907
    8. These results suggest that TNF-alpha and IL-10, but not IL-6, are involved in the late reparatory phases of the experimental disk lesion. PMID: 19605905
    9. The present study shows that elevated levels of serum CRP and IL-10 were associated with porcine circovirus type 2-infected piglets that subsequently developed severe PMWS.(postweaning multisystemic wasting syndrome) PMID: 16817761
    10. Subclinically porcine circovirus type 2 (PCV2)-infected pigs developed a transient PCV2-specific IL-10 response during the viremic phase of infection which coincided with the inversion of the IgM/IgG ratio. PMID: 17592737
    11. Twenty one polymorphisms in the IL10 gene have been found, including single nucleotide polymorphisms and insertion deletion polymorphisms. The chromosomal location has been mapped by FISH and radiation hybrid mapping. PMID: 18607788
    12. findings show that Brachyspira hyodysenteriae inoculation induced production of systemic levels of IL-1beta during the dysentery period and increased levels of IL-10 coincided with recovery from dysentery PMID: 18700003

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ssc:397106

    STRING: 9823.ENSSSCP00000016594

    UniGene: Ssc.148