DIRAS1 Antibody

說明書

中文名稱：

DIRAS1兔多克隆抗體
貨號：

CSB-PA006905LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA006905LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with CSB-PA006905LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) DIRAS1 Polyclonal antibody
Uniprot No.：

O95057
基因名：

DIRAS1
別名：

DIRAS1 antibody; GBTS1 antibody; RIGGTP-binding protein Di-Ras1 antibody; Distinct subgroup of the Ras family member 1 antibody; Ras-related inhibitor of cell growth antibody; Rig antibody; Small GTP-binding tumor suppressor 1 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human GTP-binding protein Di-Ras1 protein (1-195AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，DIRAS1 Antibody (CSB-PA006905LA01HU)，的標(biāo)記方式是Non-conjugated。對于DIRAS1 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA006905LB01HU	DIRAS1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA006905LC01HU	DIRAS1 Antibody, FITC conjugated
Biotin	CSB-PA006905LD01HU	DIRAS1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Displays low GTPase activity and exists predominantly in the GTP-bound form.
基因功能參考文獻(xiàn)：
1. This neuronal DIRAS1 gene with a proposed role in cholinergic transmission provides not only a candidate for human myoclonic epilepsy but also insights into the disease etiology, while establishing a spontaneous model for future intervention studies and functional characterization. PMID: 28223533
2. Results show that DIRAS1 is frequently methylated in human colorectal cancer and the expression of DIRAS1 is silenced by promoter region methylation. PMID: 28491151
3. DiRas1 expression inhibits malignant features of cancers in part by nonproductively binding to SmgGDS and inhibiting the binding of other small GTPases to SmgGDS PMID: 26814130
4. DIRAS1 downregulation is associated with esophageal squamous cell carcinoma metastasis. PMID: 23436800
5. a novel Ras-related protein and potential neural tumor suppressor (RIG) PMID: 12107278
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亞細(xì)胞定位：

Cell membrane; Lipid-anchor; Cytoplasmic side.
蛋白家族：

Small GTPase superfamily, Di-Ras family
組織特異性：

Highly expressed in heart and brain.
數(shù)據(jù)庫鏈接：

HGNC: 19127

OMIM: 607862

KEGG: hsa:148252

STRING: 9606.ENSP00000325836

UniGene: Hs.172753