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NUF2 Recombinant Monoclonal Antibody

  • 中文名稱：
    NUF2重組抗體
  • 貨號：
    CSB-RA248269A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate
      All lanes: NUF2 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 55 kDa
      Observed band size: 55 kDa
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA248269A0HU為NUF2重組單克隆抗體，靶向細(xì)胞周期關(guān)鍵調(diào)控蛋白NUF2。該蛋白在有絲分裂中通過與著絲粒相關(guān)復(fù)合物結(jié)合，參與紡錘體組裝及染色體分離過程，其異常表達與基因組不穩(wěn)定性及腫瘤發(fā)生密切相關(guān)。本抗體經(jīng)ELISA和Western Blot（WB）嚴(yán)格驗證，在WB實驗中可特異性識別內(nèi)源性NUF2蛋白，推薦使用稀釋度為1:500-1:2000。采用重組單抗技術(shù)制備，具備高親和力與批次一致性，適用于細(xì)胞周期調(diào)控機制研究、有絲分裂異常分析以及腫瘤模型中NUF2蛋白表達的檢測，為探索染色體穩(wěn)定性相關(guān)疾病機制提供可靠工具。該產(chǎn)品僅限于科學(xué)研究使用。
  • Uniprot No.：
  • 基因名：
    NUF2
  • 別名：
    Kinetochore protein Nuf2 (hNuf2) (hNuf2R) (hsNuf2) (Cell division cycle-associated protein 1), NUF2, CDCA1 NUF2R
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human NUF2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    8B7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
  • 基因功能參考文獻：
    1. CDCA1 could be a prognostic marker in malignant melanoma patients. PMID: 27237743
    2. Results provide evidence that CDCA1 plays a significant role in oral cavity carcinoma (OCC) tumorigenesis. Importantly, CDCA1 is associated with growth and survival of OCC cells where its elevated expression issignificantly associated with poor prognosis . PMID: 27499128
    3. NUF2 modulates cell proliferation via cell cycle control in osteosarcoma Saos-2 cells. PMID: 27049259
    4. found LncRNA AF339813 was positively regulated by NUF2 PMID: 26045769
    5. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. PMID: 25370920
    6. NUF2 plays a critical role in the regulation of HCC cell proliferation and apoptosis. PMID: 25374179
    7. NUF2 was overexpressed in glioma tissues and differentially expressed in a series of glioma cell lines. Depletion of NUF2 by short-hairpin RNA inhibited cell growth in vitro and in vivo. PMID: 25481014
    8. hnRNP K binds to the promoter region of NUF2 and activates its transcription. PMID: 25701787
    9. These results suggest that CDCA1 could be a prognostic biomarker and a potential therapeutic target for colorectal cancers . PMID: 24247253
    10. these findings demonstrated that three buried glutamic acid-lysine pairs, in concert with hydrophobic interactions of core residues, provide the major specificity and stability requirements for Hec1-Nuf2 dimerization PMID: 24129578
    11. NDC80, NUF2 and PTN were significantly aberrantly overexpressed in serous adenocarcinomas. PMID: 23056589
    12. These data suggest that the CH and tail domains of Hec1 generate essential contacts between kinetochores and microtubules in cells, whereas the Nuf2 CH domain does not. PMID: 21270439
    13. cell growths of colorectal and gastric cancers after the siRNA-mediated knockdown of either CDCA1 or KNTC2 were significantly suppressed. PMID: 19878654
    14. Data highlight a specific role for hNuf2 in kinetochore-microtubule attachment and suggest that hNuf2 is part of a molecular linker between the kinetochore attachment site and tubulin subunits within the lattice of attached plus ends. PMID: 12438418
    15. NUF2 is a core component of the kinetochore outer plate essential for organizing microtubule attachment sites. PMID: 15548592
    16. HsNUF2 and CENP-E are required for organization of stable microtubule-kinetochore attachment that is essential for faithful chromosome segregation in mitosis PMID: 17535814
    17. CDCA1 may therefore be an ideal tumor-associated antigen useful for the diagnosis and immunotherapy of these cancer PMID: 18770861
    18. alternative splicing variants, M-RIP, HYAL2, CDCA1, and MSMB genes showed differential expressions between cancer cells and corresponding normal tissues. PMID: 19081476
    19. Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization.
  • 蛋白家族：
    NUF2 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 14621

    OMIM: 611772

    KEGG: hsa:83540

    STRING: 9606.ENSP00000271452

    UniGene: Hs.651950