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  4. Recombinant Arabidopsis thaliana Light-mediated development protein DET1 (DET1), partial

Recombinant Arabidopsis thaliana Light-mediated development protein DET1 (DET1), partial

  • 中文名稱(chēng)：
    擬南芥DET1重組蛋白
  • 貨號(hào)：
    CSB-YP341784DOA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    擬南芥DET1重組蛋白
  • 貨號(hào)：
    CSB-EP341784DOA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    擬南芥DET1重組蛋白
  • 貨號(hào)：
    CSB-EP341784DOA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    擬南芥DET1重組蛋白
  • 貨號(hào)：
    CSB-BP341784DOA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    擬南芥DET1重組蛋白
  • 貨號(hào)：
    CSB-MP341784DOA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    DET1; At4g10180; F28M11.100; T9A4.13Light-mediated development protein DET1; Protein DEETIOLATED 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of light signal transduction machinery. Involved in repression of photomorphogenesis in darkness by participating in the CDD complex, a complex probably required to regulate the activity of ubiquitin conjugating enzymes (E2s). Involved in repression of deetiolation in the developing seedling. Repression of photomorphogenesis is probably mediated by ubiquitination and subsequent degradation of photomorphogenesis-promoting factors such as HY5, HYH and LAF1. Involved in the repression of blue light responsive promoter in chloroplasts. May be required to stabilize the CDD complex. Its association with histone tail suggests a role in remodeling of chromatin. Required for the regulation of histone H2B monoubiquitination (H2Bub) over most genes by controlling the stability of the deubiquitination module (DUB module).
  • 基因功能參考文獻(xiàn)：
    1. Germination in det1 mutants is sensitive to ABA. Double mutant analysis indicates that det1 abscisic acid sensitive germination requires HY5 and ABI5. PMID: 26398801
    2. DET1 is a strong repressor of flowering and has a pivotal role in maintaining photoperiod sensitivity in the regulation of flowering time. PMID: 25962685
    3. PEX2 RING domain overexpression in det1 also partially suppresses the det1 phenotype. PMID: 25248106
    4. Genomic analysis also revealed that DET1 may modulate the expression of light-regulated genes to mediate photomorphogenesis partially through phytochrome-interacting factors. PMID: 25248553
    5. DET1 acts upstream of both HFR1 and PIF1 to predominantly repress seed germination. DET1 predominantly represses seed germination in the dark. PMID: 25775589
    6. The det1 acts epistatically to uvh6 with respect to de-etiolation, chlorophyll level, UV tolerance, and heat-induced growth inhibition. (DET1 protein) PMID: 22908040
    7. results reveal DET1 as a core transcriptional repression factor important for clock progression PMID: 21884973
    8. Altogether, these data suggest that DET1, CUL4 and DDB2 cooperate during the excision repair process. PMID: 21304489
    9. LHY ubiquitination by SINAT5 was inhibited by DET1. PMID: 20599732
    10. Findings suggests a role for DET1 in a ubiquitination pathway and identify a substrate for DET1-regulated protein turn-over. PMID: 15988568
    11. Arabidopsis cullin 4 is shown to assemble with DDB1, RBX1, DET1 and DDB2 in vitro and in planta. PMID: 16792691

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  • 亞細(xì)胞定位：
    Nucleus, nucleoplasm.
  • 蛋白家族：
    DET1 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT4G10180

    STRING: 3702.AT4G10180.1

    UniGene: At.33647