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  4. Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 regulatory subunit (AXR1), partial

Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 regulatory subunit (AXR1), partial

  • 中文名稱(chēng)：
    Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 regulatory subunit(AXR1) ,partial
  • 貨號(hào)：
    CSB-YP015411DOA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 regulatory subunit(AXR1) ,partial
  • 貨號(hào)：
    CSB-EP015411DOA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 regulatory subunit(AXR1) ,partial
  • 貨號(hào)：
    CSB-EP015411DOA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 regulatory subunit(AXR1) ,partial
  • 貨號(hào)：
    CSB-BP015411DOA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 regulatory subunit(AXR1) ,partial
  • 貨號(hào)：
    CSB-MP015411DOA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    AXR1
  • Uniprot No.：
  • 別名：
    AXR1; At1g05180; YUP8H12.21; NEDD8-activating enzyme E1 regulatory subunit AXR1; Auxin-resistance protein AXR1; Protein AUXIN-RESISTANT 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Regulatory subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1. Plays an important role in auxin response. Regulates the chromosomal localization of meiotic recombination by crossovers (COs) and subsequent synapsis, probably through the activation of a CRL4 complex. Required for E3-mediated protein degradation in response to auxin, jasmonic acid and cold stress. Required for the COP1-COP10-CSN-mediated repression of photomorphogenesis in the dark. May function redundantly with AXL1 in the RUB conjugating pathway. Seems not to be functionally equivalent to AXL1 in vivo.
  • 基因功能參考文獻(xiàn)：
    1. AXR1 and other neddylation enzymes are autoneddylated in an E2- and E3-independent manner in vitro and that AXR1 autoneddylation impairs NEDD8 E2 approximately thioester formation in the den1 mutant background in vivo. PMID: 28096463
    2. SMAP1 is necessary for root meristem formation and normal embryo development in the axr1 background. PMID: 22576848
    3. Data show taht root hair cell-specific overexpression of the influx transporter AUXIN-RESISTANT1 enhanced root hair length. PMID: 18156217
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Ubiquitin-activating E1 family, ULA1 subfamily
  • 組織特異性：
    Expressed in shoot, root and floral meristems, in vascular tissues of cotyledons and mature leaves, and in the stele of the root. Expressed at higher levels on the lower side of an emerging root during germination and at higher levels on the underside of
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT1G05180

    STRING: 3702.AT1G05180.1

    UniGene: At.10217