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  4. Recombinant Arabidopsis thaliana Transcription factor VIP1 (VIP1)

Recombinant Arabidopsis thaliana Transcription factor VIP1 (VIP1)

  • 中文名稱：
    擬南芥VIP1重組蛋白
  • 貨號：
    CSB-EP878716DOA
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    VIP1
  • Uniprot No.：
  • 別名：
    Protein SULPHATE UTILIZATION EFFICIENCY 3;VirE2-interacting protein 1;AtVIP1;bZIP transcription factor 51;AtbZIP51;bZIP protein 51
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    44.7 kDa
  • 表達區(qū)域：
    1-341aa
  • 氨基酸序列
    MEGGGRGPNQTILSEIEHMPEAPRQRISHHRRARSETFFSGESIDDLLLFDPSDIDFSSLDFLNAPPPPQQSQQQPQASPMSVDSEETSSNGVVPPNSLPPKPEARFGRHVRSFSVDSDFFDDLGVTEEKFIATSSGEKKKGNHHHSRSNSMDGEMSSASFNIESILASVSGKDSGKKNMGMGGDRLAELALLDPKRAKRILANRQSAARSKERKIRYTGELERKVQTLQNEATTLSAQVTMLQRGTSELNTENKHLKMRLQALEQQAELRDALNEALRDELNRLKVVAGEIPQGNGNSYNRAQFSSQQSAMNQFGNKTNQQMSTNGQPSLPSYMDFTKRG
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcription activator that binds specifically to the VIP1 response elements (VREs) DNA sequence 5'-ACNGCT-3' found in some stress genes (e.g. TRX8 and MYB44), when phosphorylated/activated by MPK3. Required for Agrobacterium VirE2 nuclear import and tumorigenicity. Promotes transient expression of T-DNA in early stages by interacting with VirE2 in complex with the T-DNA and facilitating its translocation to the nucleus, and mediates stable genetic transformation by Agrobacterium by binding H2A histone. Prevents cell differentiation and shoot formation. Limits sulfate utilization efficiency (SUE) and sulfate uptake, especially in low-sulfur conditions. Plays a role in osmosensory response by binding to the 5'-AGCTGT/G-3' DNA sequence found in the promoters of the hypoosmolarity-responsive genes CYP707A1 and CYP707A3. Involved in the negative regulation of touch-induced root bending and salt-dependent root bending.
  • 基因功能參考文獻：
    1. These results raise the possibility that VIP1 influences structures of the root cap and thereby regulates the local auxin responses in roots. PMID: 27208231
    2. dephosphorylation of VIP1 resulting from mechanical or hypo-osmotic stress induces nuclear localization via 14-3-3 dissociation PMID: 28542772
    3. VIP1 suppresses touch-induced responses. PMID: 27171129
    4. Developed efficient protocols that resulted in pure and stable His-tagged VIP1 and VirE2, and using the purified proteins, performed peptide array screening and revealed the binding sites on both proteins. PMID: 25212215
    5. VIP1 is not important for Agrobacterium-mediated transformation or VirE2 subcellular localization. PMID: 24953893
    6. When overexpressed in Arabidopsis, some of them as well as VIP1 caused growth retardation under a mannitol-stressed condition, where VIP1 is localized mainly in the cytoplasm. PMID: 25093810
    7. Investigated the functions of VIP1, we discovered that VIP1 can react with an Ni(2)-activated derivative of horseradish peroxidase, HisProbe-HRP, suggesting that VIP1 can bind Ni(2). PMID: 24057918
    8. A VIP1 response element-containing promoter was observed in dividing cells, probably resulting from activation of endogenous VIP1. PMID: 23942522
    9. The nuclear localization of VIP1 is enhanced by increased turgor. VIP1 functions as a regulator of osmosensory signaling in Arabidopsis. PMID: 22452852
    10. Data indicate that the ability of VIP1 to interact with the VirE2 protein component of the T-complex and localize to the cell nucleus is sufficient for transient genetic transformation. PMID: 15824315
    11. provide evidence that VIP1 encodes a functional bZIP transcription factor that stimulates stress-dependent gene expression by binding to VIP1 response elements (VREs), a DNA hexamer motif. PMID: 19820165

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  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    BZIP family
  • 組織特異性：
    Mostly expressed in dividing cells, present in leaves, roots and seedlings.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT1G43700

    STRING: 3702.AT1G43700.1

    UniGene: At.22614