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  4. Recombinant Bacillus anthracis Thymidylate kinase (tmk)

Recombinant Bacillus anthracis Thymidylate kinase (tmk)

In Stock
  • 中文名稱：
    炭疽桿菌tmk重組蛋白
  • 貨號：
    CSB-EP007226BQG
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    tmk
  • Uniprot No.：
  • 別名：
    tmk; BAMEG_0039Thymidylate kinase; EC 2.7.4.9; dTMP kinase
  • 種屬：
    Bacillus anthracis (strain CDC 684 / NRRL 3495)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    27.8kDa
  • 表達(dá)區(qū)域：
    1-208aa
  • 氨基酸序列
    MKGLFVTIEGPEGSGKTTLIQSLLPYFEQKEQKVMATREPGGIAISEDIRTILHKQEYTMMEARTEALLYAAARRQHLVEKVMPALNEDYLVLCDRFIDSSLAYQGYARGLGMDKVFEINRFATEDCMPSLTIYLDIEPEVGLARIAKDAGREVNRLDMEDISFHKRVREGYLQVVERFSDRIVLVNADQPMEKLIEEVIQVIEDKLL
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The full-length cDNA ORF of Bacillus anthracis Thymidylate kinase (tmk) was N-terminally tagged a 6xHis and then expressed in E.coli. The Recombinant Bacillus anthracis tmk obtained was purified using SDS-PAGE analysis and got over 90% of purity. Under reducing conditions, the tmk protein migrated to an approximately 28 kDa molecular mass band on the SDS-PAGE gel. This recombinant tmk protein may be used to produce specific antibodies or find uses in the DNA synthesis-related studies.

    Tmk is a key enzyme exerting an important role in DNA synthesis. It catalyzes the conversion of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), with ATP as the preferred phosphoryl donor and in the presence of Mg2+. It takes place at the junction of the de novo and salvage pathways for thymidine triphosphate (dTTP) synthesis. Tmk is thus regarded as a potential therapeutic target for the development of antibacterial, antiparasitic, and anticancer drugs.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.
  • 蛋白家族：
    Thymidylate kinase family
  • 數(shù)據(jù)庫鏈接：