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  4. Recombinant Bacillus subtilis RNA polymerase sigma-F factor (sigF)

Recombinant Bacillus subtilis RNA polymerase sigma-F factor (sigF)

  • 中文名稱：
    枯草芽孢桿菌sigF重組蛋白
  • 貨號：
    CSB-YP357235BRJ
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigF重組蛋白
  • 貨號：
    CSB-EP357235BRJ
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigF重組蛋白
  • 貨號：
    CSB-EP357235BRJ-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigF重組蛋白
  • 貨號：
    CSB-BP357235BRJ
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigF重組蛋白
  • 貨號：
    CSB-MP357235BRJ
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    sigF
  • Uniprot No.：
  • 別名：
    sigF; spoIIAC; BSU23450; RNA polymerase sigma-F factor; Sporulation sigma factor; Stage II sporulation protein AC
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-255
  • 氨基酸序列
    MDVEVKKNGK NAQLKDHEVK ELIKQSQNGD QQARDLLIEK NMRLVWSVVQ RFLNRGYEPD DLFQIGCIGL LKSVDKFDLT YDVRFSTYAV PMIIGEIQRF IRDDGTVKVS RSLKELGNKI RRAKDELSKT LGRVPTVQEI ADHLEIEAED VVLAQEAVRA PSSIHETVYE NDGDPITLLD QIADNSEEKW FDKIALKEAI SDLEEREKLI VYLRYYKDQT QSEVAERLGI SQVQVSRLEK KILKQIKVQM DHTDG
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with SpoIIAB inhibits sigma-F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore. Responsible for expression of csfB (the anti-sigma-G factor Gin). Associates with the RNAP core only in stationary phase cells.
  • 基因功能參考文獻：
    1. The obtained knowledge is relevant for various experiments, such as industrial fermentation, prolonged experimental evolution or zero-growth studies, where sporulation is an undesirable trait that should be avoided, e.g by a sigF mutation. PMID: 26506528
    2. effect of SpoIIAB and SpoIIAA protein concentrations on the prespore-specific activation of sigma(F) occurring in the early stage of sporulation PMID: 20298743
    3. Early in sporulation, the cell divides asymmetrically to give a smaller prespore and a larger mother cell. Differential gene expression in these compartments depends on the regulation of the sigma F which is activated only in the prespore PMID: 15819616
    4. Developmental commitment during sporulation is governed by the transcription factors sigma(F) and sigma(E), which are activated in the forespore and the mother cell, respectively. PMID: 15882622
  • 蛋白家族：
    Sigma-70 factor family
  • 數據庫鏈接：