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  4. Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac), partial

Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac), partial

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  • 中文名稱：
    Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac(cry1Ac),partial
  • 貨號：
    CSB-EP356448BDC
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    cry1Ac
  • Uniprot No.：
  • 別名：
    cry1Ac; cry1A(c); cry218; cryIA(c)Pesticidal crystal protein Cry1Ac; 133 kDa crystal protein; Crystaline entomocidal protoxin; Insecticidal delta-endotoxin CryIA(c)
  • 種屬：
    Bacillus thuringiensis subsp. kurstaki
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    50.8kDa
  • 表達(dá)區(qū)域：
    972-1178aa
  • 氨基酸序列
    LYDARNVIKNGDFNNGLSCWNVKGHVDVEEQNNQRSVLVVPEWEAEVSQEVRVCPGRGYILRVTAYKEGYGEGCVTIHEIENNTDELKFSNCVEEEIYPNNTVTCNDYTVNQEEYGGAYTSRNRGYNEAPSVPADYASVYEEKSYTDGRRENPCEFNRGYRDYTPLPVGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal GST-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The Recombinant kurstaki cry1Ac protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of cry1Ac gene. This recombinant cry1Ac protein was expressed in the host. The expression region is 972-1178aa of the kurstaki cry1Ac. In the production, the expression vector contains N-terminal GST tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.

    The Cry1Ac protoxin, which is a delta-endotoxin produced during the sporulation phase of Bacillus thuringiensis, has been proposed as an effective and safe alternative adjuvant. When ingested by susceptible larvae, the crystal protein is solubilized and processed, generating the Cry1Ac toxin, which has been widely used as a bioinsecticide. The recombinant Cry1Ac protoxin is immunogenic and adjuvant at the systemic and mucosal level, which can activate antigen presenting cells (APCs) by upregulating costimulatory molecules and increasing the production of pro-inflammatory cytokines. It can confer protective immunity against distinct infection models and remarkably in a murine brucellosis model, it showed the capacity to induce both Th1 and TCD8+ cytotoxic lymphocyte responses, suggesting a potential utility to improve tumor immunity. Beyond this, a study found that a differential adjuvant capacity of Cry1Ac proteins to improve tumor immunity, being the effect of Cry1Ac protoxin outstanding compared with that of Cry1Ac toxin. In addition, Cry1Ac protoxin may confer therapeutic benefit when coadministered with doxorubicin.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.
  • 蛋白家族：
    Delta endotoxin family