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  4. Recombinant Bovine Protein S100-A8 (S100A8)

Recombinant Bovine Protein S100-A8 (S100A8)

  • 中文名稱：
    牛S100A8重組蛋白
  • 貨號：
    CSB-YP020641BO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛S100A8重組蛋白
  • 貨號：
    CSB-EP020641BO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛S100A8重組蛋白
  • 貨號：
    CSB-EP020641BO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛S100A8重組蛋白
  • 貨號：
    CSB-BP020641BO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛S100A8重組蛋白
  • 貨號：
    CSB-MP020641BO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    S100A8Protein S100-A8; BEE11; Calgranulin-A; Neutrophil cytosolic 7 kDa protein; P7; S100 calcium-binding protein A8
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-89
  • 氨基酸序列
    MLTDLECAIN SLIDVYHKYS LKKGNYHAVY RDDLKQLLET ECPKFMKKKD ADTWFKELDI NQDGGINFEE FLVLVIKVGL EAHEEIHKE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
    牛蛋白S100-A8（S100A8）是一種小分子的鈣調(diào)蛋白，參與了多種細胞功能的調(diào)節(jié)和信號傳導。如果想要重組這種蛋白，可以按照以下步驟進行：
    克隆S100A8基因：可以使用PCR技術(shù)從牛的基因組DNA中擴增S100A8基因的開放閱讀框（ORF）序列。此外，還需要添加一些啟動子和標簽序列，以便在表達過程中調(diào)節(jié)基因表達，并且可以方便地檢測到蛋白質(zhì)。
    構(gòu)建表達載體：將S100A8基因插入表達載體中，可以選擇一些經(jīng)過驗證的表達載體，如pET系列載體。這些載體中包含了一些促進蛋白表達的元件，如啟動子、增強子、終止子等。
    選擇表達宿主菌：可以選擇一些常用的表達宿主菌，如大腸桿菌（E. coli），并在菌株中轉(zhuǎn)化含有S100A8基因的表達載體。這些宿主菌中還需要添加一些輔助蛋白質(zhì)，如分泌蛋白、分子伴侶等，以幫助蛋白質(zhì)的正確折疊和純化。
    蛋白表達和純化：在表達宿主菌中誘導S100A8蛋白的表達，并通過細胞破碎和離心等方法提取蛋白質(zhì)。然后使用親和層析、離子交換層析、凝膠過濾層析等方法對蛋白進行純化。
    蛋白質(zhì)的鑒定和活性檢測：使用SDS-PAGE、Western blot等方法對純化后的S100A8蛋白進行鑒定和定量。此外，還可以使用細胞實驗等方法對S100A8蛋白的生物活性進行檢測，如檢測其對細胞的增殖、分化、遷移等的影響。
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.
  • 基因功能參考文獻：
    1. The findings indicated that plasma concentrations of S100A7 and S100A8 did not change significantly during pregnancy in cows. PMID: 29604144
    2. study of nonsynonymous DNA variation in the functional domain of the S100A7, -A8, -A9 genes in taurine cattle and yak PMID: 29774569
    3. Data suggest that up-regulation of S100A8 and S100A9 is a key component of early endometrial response to uterine involution in the post-partum period and to prevent chronic endometritis/uterine inflammation; up-regulation can be influenced by diet. PMID: 23533291
  • 亞細胞定位：
    Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    S-100 family
  • 組織特異性：
    Found essentially in phagocytic cells.
  • 數(shù)據(jù)庫鏈接：