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  4. Recombinant Buchnera aphidicola subsp. Schizaphis graminum Exodeoxyribonuclease V beta chain (recB), partial

Recombinant Buchnera aphidicola subsp. Schizaphis graminum Exodeoxyribonuclease V beta chain (recB), partial

  • 中文名稱：
    蚜蟲亞屬斯克拉姆松recB重組蛋白
  • 貨號：
    CSB-YP809138BXE
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    蚜蟲亞屬斯克拉姆松recB重組蛋白
  • 貨號：
    CSB-EP809138BXE
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    蚜蟲亞屬斯克拉姆松recB重組蛋白
  • 貨號：
    CSB-EP809138BXE-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    蚜蟲亞屬斯克拉姆松recB重組蛋白
  • 貨號：
    CSB-BP809138BXE
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    蚜蟲亞屬斯克拉姆松recB重組蛋白
  • 貨號：
    CSB-MP809138BXE
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    recB
  • Uniprot No.：
  • 別名：
    recB; BUsg_439; RecBCD enzyme subunit RecB; EC 3.1.11.5; Exonuclease V subunit RecB; ExoV subunit RecB; Helicase/nuclease RecBCD subunit RecB
  • 種屬：
    Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.
  • 蛋白家族：
    Helicase family, UvrD subfamily
  • 數(shù)據(jù)庫鏈接：