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  4. Recombinant Chicken Serine/threonine-protein kinase B-raf (BRAF), partial

Recombinant Chicken Serine/threonine-protein kinase B-raf (BRAF), partial

  • 中文名稱：
    Recombinant Chicken Serine/threonine-protein kinase B-raf(BRAF) ,partial
  • 貨號(hào)：
    CSB-YP002791CH
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Chicken Serine/threonine-protein kinase B-raf(BRAF) ,partial
  • 貨號(hào)：
    CSB-EP002791CH
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Chicken Serine/threonine-protein kinase B-raf(BRAF) ,partial
  • 貨號(hào)：
    CSB-EP002791CH-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Chicken Serine/threonine-protein kinase B-raf(BRAF) ,partial
  • 貨號(hào)：
    CSB-BP002791CH
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Chicken Serine/threonine-protein kinase B-raf(BRAF) ,partial
  • 貨號(hào)：
    CSB-MP002791CH
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    BRAF; RMIL; Serine/threonine-protein kinase B-raf; EC 2.7.11.1; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; Serine/threonine-protein kinase Rmil
  • 種屬：
    Gallus gallus (Chicken)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Protein kinase involved in the activation of the MAP signaling cascade. May play a role in transducing specific signals in neural cells.
  • 基因功能參考文獻(xiàn)：
    1. BRAF alternative splicing is differentially regulated in avian, rodent and primates. Exon 9b is present in vertebrates but exon 8b is present only in eutherians. PMID: 21203559
    2. Neutralizing mutations of D447/8, S446 & S447, or uncoupling of B-Raf from Ras-GTP, reduce chicken B-Raf actvity in PC12 differentiation. S365 is a 14-3-3 binding site. B-Raf(V600E) cannot be inhibited by blocking S446 phosphorylation or Ras activation. PMID: 16702958
    3. Inducible gene deletion of the c-Rmil/B-raf gene in chicken DT40 B cells revealed that B-Raf is required for the maintenance of basal ERK activity as well as for a rapid and sustained ERK activation following B cell antigen receptor (BCR) activation. PMID: 12411479
  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm. Cell membrane.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
  • 組織特異性：
    Expressed preferentially in neural tissue.
  • 數(shù)據(jù)庫鏈接：