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  4. Recombinant Chlamydia trachomatis serovar L2 Major outer membrane porin (ompA)

Recombinant Chlamydia trachomatis serovar L2 Major outer membrane porin (ompA)

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  • 中文名稱：
    Recombinant Chlamydia trachomatis serovar L2 Major outer membrane porin(ompA)
  • 貨號：
    CSB-EP356881DSG
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356881DSG could indicate that this peptide derived from E.coli-expressed Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) ompA.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP356881DSG could indicate that this peptide derived from E.coli-expressed Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) ompA.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ompA
  • Uniprot No.：
  • 別名：
    ompA; omp1L2; CTL0050; Major outer membrane porin; MOMP
  • 種屬：
    Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    56.3kDa
  • 表達區(qū)域：
    23-394aa
  • 氨基酸序列
    LPVGNPAEPSLMIDGILWEGFGGDPCDPCTTWCDAISMRMGYYGDFVFDRVLQTDVNKEFQMGAKPTTATGNAAAPSTCTARENPAYGRHMQDAEMFTNAAYMALNIWDRFDVFCTLGATSGYLKGNSASFNLVGLFGDNENHATVSDSKLVPNMSLDQSVVELYTDTTFAWSAGARAALWECGCATLGASFQYAQSKPKVEELNVLCNAAEFTINKPKGYVGQEFPLDLKAGTDGVTGTKDASIDYHEWQASLALSYRLNMFTPYIGVKWSRASFDADTIRIAQPKSATTVFDVTTLNPTIAGAGDVKASAEGQLGDTMQIVSLQLNKMKSRKSCGIAVGTTIVDADKYAVTVETRLIDERAAHVNAQFRF
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    In the production of recombinant C.trachomatis ompA protein, the gene for ompA (E.coli) was cloned into a vector and expressed as ompA protein in E.coli. The plasmids with the copy of ompA, or the expression vector, were often used to enhance gene expression. Every step of production was undergone with a strict QC system. N-terminal 6xHis-SUMO tag was used in the process. The purity is 90% determined by SDS-PAGE.

    OmpA was first identified as a heat-modifiable protein in Escherichia coli (E. coli) in 1974 and originally purified in 1977. Its molecular mass ranges from 28 kDa to 36 kDa. OmpA family is a group of surface-exposed, porin proteins with high-copy number in Omps of GNB. N-terminal domain of OmpA is an antiparallel β-barrel structure consisting of eight transmembrane strands so as to be embedded in the outer membrane. Among those OMPs of A. baumannii, OmpA is the most deeply studied virulence factor which plays key roles in regulating the adhesion, aggressiveness, and biofilm formation of A. baumannii and immune response of host. Overproduction of OmpA is an independent risk factor for the mortality rate of nosocomial pneumonia and bacteremia caused by A. baumannii. Recent studies suggested that OmpA plays a key role in maintaining bacterial surface integrity. Generally, in GNB species, OmpA stabilize their structure by self-dimerization, which prevents the bending connections between βbarrel structure and periplasmic from being lysed.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the large cysteine-rich periplasmic protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan.; Permits diffusion of specific solutes through the outer membrane.
  • 亞細胞定位：
    Cell outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Chlamydial porin (CP) (TC 1.B.2) family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ctb:CTL0050