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  4. Recombinant Clostridium perfringens Perfringolysin O (pfo)

Recombinant Clostridium perfringens Perfringolysin O (pfo)

In Stock
  • 中文名稱(chēng)：
    產(chǎn)氣莢膜梭狀芽胞桿菌pfo重組蛋白
  • 貨號(hào)：
    CSB-EP314820CMB
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP314820CMB could indicate that this peptide derived from E.coli-expressed Clostridium perfringens (strain 13 / Type A) pfo.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP314820CMB could indicate that this peptide derived from E.coli-expressed Clostridium perfringens (strain 13 / Type A) pfo.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    pfo
  • Uniprot No.：
  • 別名：
    pfo; pfoA; pfoR; CPE0163; Perfringolysin O; Theta-toxin; Thiol-activated cytolysin
  • 種屬：
    Clostridium perfringens (strain 13 / Type A)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 來(lái)源：
    E.coli
  • 分子量：
    68.7kDa
  • 表達(dá)區(qū)域：
    29-500aa
  • 氨基酸序列
    KDITDKNQSIDSGISSLSYNRNEVLASNGDKIESFVPKEGKKTGNKFIVVERQKRSLTTSPVDISIIDSVNDRTYPGALQLADKAFVENRPTILMVKRKPININIDLPGLKGENSIKVDDPTYGKVSGAIDELVSKWNEKYSSTHTLPARTQYSESMVYSKSQISSALNVNAKVLENSLGVDFNAVANNEKKVMILAYKQIFYTVSADLPKNPSDLFDDSVTFNDLKQKGVSNEAPPLMVSNVAYGRTIYVKLETTSSSKDVQAAFKALIKNTDIKNSQQYKDIYENSSFTAVVLGGDAQEHNKVVTKDFDEIRKVIKDNATFSTKNPAYPISYTSVFLKDNSVAAVHNKTDYIETTSTEYSKGKINLDHSGAYVAQFEVAWDEVSYDKEGNEVLTHKTWDGNYQDKTAHYSTVIPLEANARNIRIKARECTGLAWEWWRDVISEYDVPLTNNINVSIWGTTLYPGSSITYN
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
        Recombinant Clostridium perfringens Perfringolysin O (pfo)是一種經(jīng)重組制備的產(chǎn)氣莢膜梭菌（Clostridium perfringens）溶原性毒素O蛋白。Perfringolysin O是產(chǎn)氣莢膜梭菌的一個(gè)主要毒素，它能夠破壞目標(biāo)細(xì)胞的細(xì)胞膜，導(dǎo)致細(xì)胞死亡。Perfringolysin O的研究對(duì)于了解產(chǎn)氣莢膜梭菌感染機(jī)制和溶原性毒素的生物學(xué)特性具有重要意義。
        華美生物的重組pfo蛋白，由大腸桿菌表達(dá)系統(tǒng)表達(dá)，具有良好的穩(wěn)定性和純度，可以更好幫助您研究其在細(xì)胞毒性和感染過(guò)程中的作用機(jī)制，以及與宿主細(xì)胞相互作用的方式；可以被廣泛應(yīng)用于生物醫(yī)學(xué)研究中，如細(xì)胞毒性實(shí)驗(yàn)、毒力機(jī)制研究和免疫學(xué)研究等。
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
  • 亞細(xì)胞定位：
    Secreted. Host cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Thiol-activated cytolysin family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: cpe:CPE0163