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  4. Recombinant Deinococcus radiodurans Protein RecA (recA)

Recombinant Deinococcus radiodurans Protein RecA (recA)

  • 中文名稱：
    放射性紅球菌recA重組蛋白
  • 貨號：
    CSB-YP331539DJA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    放射性紅球菌recA重組蛋白
  • 貨號：
    CSB-EP331539DJA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    放射性紅球菌recA重組蛋白
  • 貨號：
    CSB-EP331539DJA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    放射性紅球菌recA重組蛋白
  • 貨號：
    CSB-BP331539DJA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    放射性紅球菌recA重組蛋白
  • 貨號：
    CSB-MP331539DJA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    recA
  • Uniprot No.：
  • 別名：
    recA; DR_2340; Protein RecA; Recombinase A
  • 種屬：
    Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-363
  • 氨基酸序列
    MSKDATKEIS APTDAKERSK AIETAMSQIE KAFGKGSIMK LGAESKLDVQ VVSTGSLSLD LALGVGGIPR GRITEIYGPE SGGKTTLALA IVAQAQKAGG TCAFIDAEHA LDPVYARALG VNTDELLVSQ PDNGEQALEI MELLVRSGAI DVVVVDSVAA LTPRAEIEGD MGDSLPGLQA RLMSQALRKL TAILSKTGTA AIFINQVREK IGVMYGNPET TTGGRALKFY ASVRLDVRKI GQPTKVGNDA VANTVKIKTV KNKVAAPFKE VELALVYGKG FDQLSDLVGL AADMDIIKKA GSFYSYGDER IGQGKEKTIA YIAERPEMEQ EIRDRVMAAI RAGNAGEAPA LAPAPAAPEA AEA
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage. Probably involved in base excision repair.; Following severe irradiation (7 kGy of gamma irradiation) genomic DNA is fragmented. DNA is progressively degraded for the first 1.5 hours after IR, in a step promoted by RecA and counterbalanced by DNA Pol I and Pol III, followed by massive DNA synthesis and genome reassembly in the next hour. Optimal priming of DNA synthesis requires both RecA and RadA, Pol III initiates DNA synthesis while both Pol I and Pol III are required for its contination. In the absence of RecA the majority of the chromosome is still reconstituted, via either single-strand annealing or non-homologous end joining.
  • 基因功能參考文獻：
    1. findings suggest that phosphorylation of DrRecA enables the recombinase to selectively use abundant dsDNA substrate present during post-irradiation recovery for efficient DSB repair, thereby promoting the extraordinary radioresistance of D. radiodurans. PMID: 27255712
    2. our results suggest a major role of single strand annealing in DNA repeat deletion events in bacteria devoid of the RecA protein, and also in recA+ bacteria exposed to ionizing radiation. PMID: 26517555
    3. Data report that the N terminus of the type III secretion system translocator proteins EspB, EspD, and EspA mediate protein secretion and translocation from wild-type enteropathogenic Escherichia coli. PMID: 20418393
    4. Results describe the crystal structure of Deinococcus radiodurans RecA in complex with ATPgammaS at 2.5 angstrom resolution. PMID: 15544805
    5. effect of RecA concentration on the kinetics of DNA double-strand break repair, replication reinitiation, and LexA cleavage after gamma-irradiation PMID: 16359339

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    RecA family
  • 數(shù)據(jù)庫鏈接：

    KEGG: dra:DR_2340

    STRING: 243230.DR_2340