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  4. Recombinant Drosophila melanogaster 14-3-3 protein zeta (14-3-3zeta)

Recombinant Drosophila melanogaster 14-3-3 protein zeta (14-3-3zeta)

  • 中文名稱：
    Recombinant Drosophila melanogaster 14-3-3 protein zeta(14-3-3zeta)
  • 貨號：
    CSB-YP326879DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster 14-3-3 protein zeta(14-3-3zeta)
  • 貨號：
    CSB-EP326879DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster 14-3-3 protein zeta(14-3-3zeta)
  • 貨號：
    CSB-EP326879DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster 14-3-3 protein zeta(14-3-3zeta)
  • 貨號：
    CSB-BP326879DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster 14-3-3 protein zeta(14-3-3zeta)
  • 貨號：
    CSB-MP326879DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    14-3-3zeta
  • Uniprot No.：
  • 別名：
    14-3-3zeta; 14-3-3; 14-3-3EZ; leo; THAP; CG1787014-3-3 protein zeta; 14-3-3-like protein; Protein Leonardo
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-248
  • 氨基酸序列
    MSTVDKEELV QKAKLAEQSE RYDDMAQAMK SVTETGVELS NEERNLLSVA YKNVVGARRS SWRVISSIEQ KTEASARKQQ LAREYRERVE KELREICYEV LGLLDKYLIP KASNPESKVF YLKMKGDYYR YLAEVATGDA RNTVVDDSQT AYQDAFDISK GKMQPTHPIR LGLALNFSVF YYEILNSPDK ACQLAKQAFD DAIAELDTLN EDSYKDSTLI MQLLRDNLTL WTSDTQGDEA EPQEGGDN
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Required in Raf-dependent cell proliferation and photoreceptor differentiation during eye development. Acts upstream of Raf and downstream of Ras, and is essential for viability. Acts as a negative regulator of the slo calcium channel via its interaction with slo-binding protein slob. Inhibits yki activity by restricting its nuclear localization. Binds to and promotes the activity of phosphoinositide 3-kinase Pi3K68D which converts phosphatidylinositol to phosphatidylinositol-3-phosphate and promotes maturation of early endosomes.
  • 基因功能參考文獻(xiàn)：
    1. Study suggests a novel mechanism of Tor regulation mediated by 14-3-3 interaction with Tctp and Rheb. PMID: 27151460
    2. The Khc73 stalk/14-3-3/NudE pathway defines a physical connection that coordinates the activities of multiple motor proteins to precisely position the spindle. PMID: 23987511
    3. Hpo signaling inhibited Yki nuclear localization and activity by phosphorylating Yki and both isoforms of 14-3-3, 14-3-3varepsilon and 14-3-3zeta, regulate Yki activity through modulating its subcellular localization. PMID: 19900439
    4. in vivo 14-3-3zeta monomer properties and functionality PMID: 19920133
    5. monomeric D14-3-3zeta is capable of modulating dSlo channel activity in this regulatory complex. PMID: 12529354
    6. These observations provide the first direct evidence that a 14-3-3 protein functions as a stress-induced molecular chaperone that dissolves and renaturalizes thermal-aggregated proteins. PMID: 16943323
    7. Results report a bona fide third functional isoform encoded by leo divergent from the other two in structurally and functionally significant areas. PMID: 19665025

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  • 亞細(xì)胞定位：
    Cytoplasm. Early endosome.
  • 蛋白家族：
    14-3-3 family
  • 組織特異性：
    Predominantly expressed in the ventral nerve cord of the embryo, and in the neural tissues of the head. Also found in the region posterior to the morphogenetic furrow of the eye imaginal disk where cells differentiate as photoreceptors.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG17870

    STRING: 7227.FBpp0087500

    UniGene: Dm.4705