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  4. Recombinant Drosophila melanogaster Peptidoglycan-recognition protein SC2 (PGRP-SC2)

Recombinant Drosophila melanogaster Peptidoglycan-recognition protein SC2 (PGRP-SC2)

In Stock
  • 中文名稱：
    黑腹果蠅PGRP-SC2重組蛋白
  • 貨號：
    CSB-EP892684DLU
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    PGRP-SC2
  • Uniprot No.：
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    25.2 kDa
  • 表達區(qū)域：
    21-184aa
  • 氨基酸序列
    VTIISKSEWGGRSATSKTSLANYLSYAVIHHTAGNYCSTKAACITQLQNIQAYHMDSLGWADIGYNFLIGGDGNVYEGRGWNVMGAHATNWNSKSIGISFLGNYNTNTLTSAQITAAKGLLSDAVSRGQIVSGYILYGHRQVGSTECPGTNIWNEIRTWSNWKA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

實驗應用：體外酶活性檢測

產(chǎn)品評價： 我使用CSB-EP892684DLU的PGRP-SC2蛋白，進行了體外酶活性檢測實驗，實驗結果表明純化的PGRP-SC2蛋白可以有效的發(fā)揮其酰胺酶活性。該公司生產(chǎn)、純化的果蠅PGRP-SC2蛋白非常好用。

By 陳老師

靶點詳情

  • 功能：
    N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity.
  • 基因功能參考文獻：
    1. The aging intestine of Drosophila, chronic activation of the transcription factor Foxo reduces expression of peptidoglycan recognition protein SC2 (PGRP-SC2), a negative regulator of IMD/Relish innate immune signaling, and homolog of the anti-inflammatory molecules PGLYRP1-4. PMID: 24439372
    2. Report that PGRP-SC1/2-depleted flies present over-activation of the immune deficiency signaling pathway after bacterial challenge suggesting that these proteins act in the larval gut to prevent activation of this pathway following bacterial ingestion. PMID: 16518472
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    N-acetylmuramoyl-L-alanine amidase 2 family
  • 組織特異性：
    Constitutively expressed at high level in gut, in addition to the induced expression in fat body.
  • 數(shù)據(jù)庫鏈接：