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  4. Recombinant Drosophila melanogaster Protein hunchback (hb), partial

Recombinant Drosophila melanogaster Protein hunchback (hb), partial

  • 中文名稱：
    黑腹果蠅hb重組蛋白
  • 貨號：
    CSB-YP361460DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅hb重組蛋白
  • 貨號：
    CSB-EP361460DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅hb重組蛋白
  • 貨號：
    CSB-EP361460DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅hb重組蛋白
  • 貨號：
    CSB-BP361460DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅hb重組蛋白
  • 貨號：
    CSB-MP361460DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    hb
  • Uniprot No.：
  • 別名：
    CG9786; Dmel\CG9786; hb; HUNB_DROME; Protein hunchback; R pbx; Regulator of postbithorax; Rg bx; Rg pbx
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Gap class segmentation protein that controls development of head structures.
  • 基因功能參考文獻(xiàn)：
    1. Hunchback (Hb) regulates a significant number of genes that are expressed during late differentiation stages. Hb is expressed in two polyploid subperineurial glia cells (carpet cells) and a thorough functional analysis shows that loss of Hb function results in a loss of carpet cells in the eye-antennal disc. PMID: 29360820
    2. Study concludes that hunchback (Hb) functions in progenitors (neuroblasts/GMCs) to establish heritable neuronal identity that is maintained by a Hb-independent mechanism. PMID: 28137283
    3. Authors developed a simple model of hb transcription mid-blastula transition (MBT); model provides a clear dynamical picture of hb transcription regulation as one of the many, actively regulated events concurrently taking place during the MBT. PMID: 27172244
    4. Hb bifunctionally regulates eve stripe 7, but it executes these two activities on different pieces of regulatory DNA--it activates the eve2+7 enhancer and represses the eve3+7 enhancer PMID: 25564665
    5. The expression of Hunchback in the neuroblast increases over time and the absolute levels of its expression specify two different Crustacean-cardioactive-peptide neuronal fates. PMID: 25344076
    6. The stripe enhancer is crucial for establishing the definitive border of the anterior Hb expression pattern during embryogenesis. PMID: 23122844
    7. The nested DNA elements contained in HG4-1, HG4-3, and HG4-7 all possess the cis-regulatory DNA necessary for proper temporal hunchback expression in newly-formed neuroblasts. PMID: 22033538
    8. Dan/Danr and Svp act in parallel pathways to limit Hb expression and allow neuroblasts to transition from making early-born neurons to late-born neurons at the proper time. PMID: 21429984
    9. The present results suggest that hunchback is essential for male-typical shaping of the contralateral neurites by fruitless gene. PMID: 21471381
    10. WT (self-regulatory) Hb output noise is predominantly dependent on the transcription and translation dynamics of its own expression, rather than on Bcd fluctuations. PMID: 21304932
    11. Data propose that Hunchback maintains early neuroblast competence by silencing a suite of late-expressed genes. PMID: 20335359
    12. an important player in sequential cell fate specification within the Drosophila CNS. PMID: 11861485
    13. homologous domains from the TRPS-1 and Drosophila Hunchback proteins support homodimerization, but not heterodimerization with Ikaros PMID: 12620233
    14. Results suggest that the differential regulation of Hunchback is achieved by a mechanism that integrates information created by the asymmetric distribution of Seven-up and Prospero. PMID: 16396905
    15. At low concentrations, Hb activates gene expression, whereas at high concentrations it mediates repression in the Drosophila embryo. PMID: 18287046
    16. quantified the dynamics of hb gene expression in flies that were wild-type, were mutant for hb self-regulation or Bcd binding, or contained an artificial promoter construct consisting of six Bcd and two Hb sites PMID: 18818726

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Hunchback C2H2-type zinc-finger protein family
  • 組織特異性：
    In embryo, expression of maternal transcript is highest in anterior region. Zygotic transcript is expressed in anterior region until the beginning of gastrulation and in posterior region until early gastrulation. After this, it is expressed in developing
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG9786

    STRING: 7227.FBpp0081431

    UniGene: Dm.1105