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  4. Recombinant Drosophila melanogaster Serine/threonine-protein phosphatase beta isoform (flw)

Recombinant Drosophila melanogaster Serine/threonine-protein phosphatase beta isoform (flw)

  • 中文名稱：
    黑腹果蠅flw重組蛋白
  • 貨號：
    CSB-YP344529DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅flw重組蛋白
  • 貨號：
    CSB-EP344529DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅flw重組蛋白
  • 貨號：
    CSB-EP344529DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅flw重組蛋白
  • 貨號：
    CSB-BP344529DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅flw重組蛋白
  • 貨號：
    CSB-MP344529DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    flw
  • Uniprot No.：
  • 別名：
    flw; PP1-9C; PP1-BETA-9C; CG2096; Serine/threonine-protein phosphatase beta isoform; EC 3.1.3.16; Protein flap wing
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-330
  • 氨基酸序列
    MGDFDLNVDS LIQRLLEMRS CRTGKQVQMT EAEVRGLCLK SREIFLQQPI LLELEAPLII CGDIHGQYTD LLRLFEYGGF PPAANYLFLG DYVDRGKQSL ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRYNVKLWKT FTDCFNCLPV AAIIDEKIFC CHGGLSPDLQ GMEQIRRLMR PTDVPDTGLL CDLLWSDPDK DVQGWGENDR GVSFTFGVDV VSKFLNRHEL DLICRAHQVV EDGYEFFARR QLVTLFSAPN YCGEFDNAGG MMTVDDTLMC SFQILKPSEK KAKYLYSGMN SSRPTTPQRS APMLATNKKK
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for cell adhesion in non-muscle tissues and in maintenance of muscle attachment. Vital for larval development.
  • 基因功能參考文獻(xiàn)：
    1. Flw functions together with myosin phosphatase 1beta to negatively regulate myosin activity during ring canal formation in oogenesis. PMID: 23936219
    2. identified a loss-of-function mutation of flapwing, which encodes the catalytic subunit of protein phosphatase 1beta (PP1beta) that disrupts oocyte polarization PMID: 21490061
    3. nuclear inhibitor of protein phosphatase type 1 (NIPP1) and protein phosphatase type 1 (PP1c) interact in vivo in Drosophila PMID: 12358598
    4. Data show that Drosophila PP1beta, a specific isoform of serine/threonine protein phosphatase 1 (PP1), regulates nonmuscle myosin and that this is the essential role of PP1beta. PMID: 15269282
    5. a Jun N-terminal kinase (JNK) mutant suppresses the semilethality of a strong flw allele PMID: 17277363

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  • 蛋白家族：
    PPP phosphatase family, PP-1 subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG2096

    STRING: 7227.FBpp0071381

    UniGene: Dm.7222