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  4. Recombinant Drosophila melanogaster Transcription factor collier (kn), partial

Recombinant Drosophila melanogaster Transcription factor collier (kn), partial

  • 中文名稱：
    黑腹果蠅kn重組蛋白
  • 貨號(hào)：
    CSB-YP348728DLU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅kn重組蛋白
  • 貨號(hào)：
    CSB-EP348728DLU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅kn重組蛋白
  • 貨號(hào)：
    CSB-EP348728DLU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅kn重組蛋白
  • 貨號(hào)：
    CSB-BP348728DLU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅kn重組蛋白
  • 貨號(hào)：
    CSB-MP348728DLU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    kn
  • Uniprot No.：
  • 別名：
    kn; col; CG10197Transcription factor collier; Transcription factor knot
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May act as a 'second-level regulator' of head patterning. Required for establishment of the PS(-1)/PS0 parasegmental border and formation of the intercalary segment. Required for expression of the segment polarity genes hedgehog, engrailed and wingless, and the segment-identity genes CAP and collar in the intercalary segment. Required at the onset of the gastrulation for the correct formation of the mandibular segment.
  • 基因功能參考文獻(xiàn)：
    1. two independent sites of Collier/Early B Cell Factor expression, hematopoietic progenitors and the PSC, achieve control of hematopoiesis PMID: 26866694
    2. Col direct control of expression of the transcription regulators apterous and eyes-absent is critical to specifying neuronal identities. cross-regulation between col and eya in muscle progenitor cells is required for specification of muscle identity PMID: 26204530
    3. Col expression is required in the prohemocytes to prevent their differentiation. PMID: 26150488
    4. Collier controls hemocyte homeostasis via coordinate regulation of PSC cell number and PSC signaling to prohemocytes. PMID: 22331866
    5. Second order regulator Collier directly controls intercalary-specific segment polarity gene expression. PMID: 22005665
    6. Connecting Hh, Dpp and EGF signalling in patterning of the Drosophila wing; the pivotal role of this protein in the AP organizer PMID: 12183378
    7. collier has a role in the cellular immune response to parasitization in Drosophila PMID: 15314643
    8. two apparently functionally redundant blocks of sequences for repression by UBX PMID: 15753212
    9. Kn exerts its cell-autonomous function to control the formation, and possibly the function, of class IV-like elaborated dendritic arbors. PMID: 17825045
    10. Characterization of the col upstream region driving DA3 muscle specific expression revealed the existence of three separate phases of cis-regulation, correlating with conserved binding sites for different mesodermal transcription factors. PMID: 18003742
    11. Knot increases dendritic arbor outgrowth through promoting the expression of Spastin, a microtubule-severing protein in Drosophila. PMID: 18093520
    12. Control of multidendritic neuron differentiation in Drosophila: the role of kn. PMID: 18234173

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    COE family
  • 組織特異性：
    Its expression at the blastoderm stage is restricted to a single stripe of cells corresponding to part of the intercalary and mandibular segment primordia, possibly parasegment O.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: dme:Dmel_CG10197

    STRING: 7227.FBpp0111722

    UniGene: Dm.1803