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  4. Recombinant Enterobacteria phage MS2 Capsid protein

Recombinant Enterobacteria phage MS2 Capsid protein

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  • 中文名稱：
    Recombinant Enterobacteria phage MS2 Capsid protein
  • 貨號(hào)：
    CSB-EP365998ECZ
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    Capsid protein; CP; Coat protein
  • 種屬：
    Escherichia phage MS2 (Bacteriophage MS2)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    21.2 kDa
  • 表達(dá)區(qū)域：
    2-130aa
  • 氨基酸序列
    ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIY
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer，50% glycerol
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
        Recombinant Escherichia phage MS2 Capsid protein是一種經(jīng)重組制備的大腸桿菌噬菌體MS2殼蛋白。噬菌體MS2是一種常見的RNA噬菌體，其殼蛋白在噬菌體結(jié)構(gòu)中起著重要的保護(hù)作用。作為噬菌體的一部分，MS2殼蛋白可以通過基因工程技術(shù)合成大量的重組蛋白。由于其高度保守的結(jié)構(gòu)，重組的MS2殼蛋白被廣泛應(yīng)用于研究中，如生物技術(shù)、藥物遞送和基因傳遞等領(lǐng)域。
        重組MS2殼蛋白通常用作納米顆粒載體，可以通過改變表面展示的配體或抗體，實(shí)現(xiàn)多種目的，如分子識(shí)別、藥物傳遞和基因傳遞。華美生物提供大腸桿菌表達(dá)的全長重組MS2殼蛋白，可用于研究病毒感染機(jī)制、病毒進(jìn)化和宿主免疫應(yīng)答等領(lǐng)域。
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 26 nm in diameter, and consisting of 89 capsid proteins dimers (178 capsid proteins). Involved in viral genome encapsidation through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome. The capsid contains also 1 copy of the A2 maturation protein.; Acts as a translational repressor of viral replicase synthesis late in infection. This latter function is the result of capsid protein interaction with an RNA hairpin which contains the replicase ribosome-binding site.
  • 基因功能參考文獻(xiàn)：
    1. When the MS2 capsid assembles, the conformation of bound RNA and its interaction with the PRR1 coat-protein dimer are similar in both small RNA phages PRR1 and phage MS2. PMID: 23519411
    2. A new concept in virus biology provides predictive information on structural constraints on coat protein and genome topography, revealing a previously unrecognized structural interdependence of the shapes and sizes of different viral components. PMID: 23403965
    3. This study shows that translational repressor binding results in self-association of asymmetric (AB) dimers being inhibited, whilst association of AB with symmetric (CC) dimers is greatly enhanced. PMID: 20562027
    4. MS2 coat protein binds several Escherichia coli mRNAs in vitro PMID: 11058143
  • 亞細(xì)胞定位：
    Virion.
  • 蛋白家族：
    Levivirus capsid protein family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:1260899