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  4. Recombinant Escherichia coli Aerobic respiration control protein ArcA (arcA)

Recombinant Escherichia coli Aerobic respiration control protein ArcA (arcA)

  • 中文名稱：
    Recombinant Escherichia coli Aerobic respiration control protein ArcA (arcA)
  • 貨號(hào)：
    CSB-EP359331ENV
  • 規(guī)格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    arcA
  • Uniprot No.：
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    31.4 kDa
  • 表達(dá)區(qū)域：
    1-238aa
  • 氨基酸序列
    MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYKLPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYRFCGDLED
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Member of the two-component regulatory system ArcB/ArcA. Represses a wide variety of aerobic enzymes under anaerobic conditions. Controls the resistance of E.coli to dyes; required for expression of the alkaline phosphatase and sex factor F genes; It also may be involved in the osmoregulation of envelope proteins. When activated by ArcB, it negatively regulates the expression of genes of aerobic function. Activates the transcription of the plfB operon by binding to its promoter.
  • 基因功能參考文獻(xiàn)：
    1. The combined results indicate that ArcA controls metabolism, chemotaxis, and motility contributing to the pathogenicity of Avian pathogenic Escherichia coli PMID: 26099584
    2. We propose that this plasticity in ArcA binding site architecture provides both an efficient means of encoding binding sites for ArcA, sigma(70)-RNAP and perhaps other transcription factors PMID: 24146625
    3. The deletion of arcA results in a decrease of repression on transcription of tricarboxylic acid cycle genes under glucose abundant conditions, without significantly affecting the glyoxylate pathway activity. PMID: 21481254
    4. the activity of the regulator ArcA correlated with aerobiosis but not with the redox state of the ubiquinone pool, challenging the idea that ArcA activity is inhibited by oxidized ubiquinone. PMID: 21252224
    5. fadH promoter is regulated by three independent regulators, the fatty acid degradation FadR repressor, the oxygen-responsive ArcA-ArcB two-component system, and the cyclic AMP receptor protein-cyclic AMP. PMID: 20622065
    6. The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool. PMID: 19933363
    7. Knockout of ArcA-dependent redox regulation, however, increased the aerobic tricarboxylic acid (TCA) cycle activity by over 60%. PMID: 15838044
    8. ArcB/ArcA/RssB system is involved in sigmaS induction during entry into starvation conditions. PMID: 16291649
    9. must be regulatory factors other than ArcB interacting with ArcA to control flagella genes PMID: 17213678
    10. Activation of ArcA facilitates pIV-dependent induction of psp expression. PMID: 19682256
    11. These data show that the acetyl phosphate dependent ArcA phosphorylation route does not seem to play a significant role under physiological conditions. PMID: 19851741

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 數(shù)據(jù)庫鏈接：