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  4. Recombinant Escherichia coli DNA gyrase subunit B (gyrB), partial

Recombinant Escherichia coli DNA gyrase subunit B (gyrB), partial

  • 中文名稱：
    大腸桿菌gyrB重組蛋白
  • 貨號(hào)：
    CSB-YP360158ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌gyrB重組蛋白
  • 貨號(hào)：
    CSB-EP360158ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌gyrB重組蛋白
  • 貨號(hào)：
    CSB-BP360158ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌gyrB重組蛋白
  • 貨號(hào)：
    CSB-MP360158ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    gyrB
  • Uniprot No.：
  • 別名：
    gyrB; acrB; cou; himB; hisU; nalC; parA; pcbA; b3699; JW5625DNA gyrase subunit B; EC 5.6.2.2; Type IIA topoisomerase subunit GyrB
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner to maintain chromosomes in an underwound state. This makes better substrates for topoisomerase 4 (ParC and ParE) which is the main enzyme that unlinks newly replicated chromosomes in E.coli. Gyrase catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes. Relaxes negatively supercoiled DNA in an ATP-independent manner. E.coli gyrase has higher supercoiling activity than other characterized bacterial gyrases; at comparable concentrations E.coli gyrase introduces more supercoils faster than M.tuberculosis gyrase, while M.tuberculosis gyrase has higher decatenation than supercoiling activity compared to E.coli. E.coli makes 15% more negative supercoils in pBR322 plasmid DNA than S.typhimurium; the S.typhimurium GyrB subunit is toxic in E.coli, while the E.coli copy can be expressed in S.typhimurium even though the 2 subunits have 777/804 residues identical. The enzymatic differences between E.coli gyrase and topoisomerase IV are largely due to the GyrA C-terminal domain (approximately residues 524-841) and specifically the GyrA-box.
  • 基因功能參考文獻(xiàn)：
    1. Among nalidixic acid resistant isolates...1 (2%) isolate showed gyrB (A660-T660) SNP. PMID: 27496516
    2. The GyrB subunit from Escherichia coli has been investigated, namely the ATP binding pocket both considering the protein without ligands and bound with the inhibitors clorobiocin, novobiocin and 5'-adenylyl-beta-gamma-imidodiphosphate. PMID: 21216167
    3. ParA binds DNA non-specifically in the presence of ATP. PMID: 20659294
    4. The insert domain of gyrB may have two functions, acting as a steric buttress to pre-configure the primary DNA-binding site, and serving as a relay that may help coordinate communication between different functional domains. PMID: 20675723
    5. double role played by metal ions in the catalytic steps involving DNA gyrase B PMID: 16223508
    6. These results reveal an important function for Par systems in plasmid DNA segregation that is likely to be conserved in bacteria. PMID: 18208495

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Type II topoisomerase family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ecj:JW5625

    STRING: 316407.85676345