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  4. Recombinant Escherichia coli Major curlin subunit (csgA), partial

Recombinant Escherichia coli Major curlin subunit (csgA), partial

In Stock
  • 貨號(hào)：
    CSB-EP340967ENV1
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    csgA
  • Uniprot No.：
  • 種屬：
    Escherichia coli(strain K12)
  • 蛋白長(zhǎng)度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    16.6 kDa
  • 表達(dá)區(qū)域：
    45-134aa
  • 氨基酸序列
    LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSS
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Curlin is the structural subunit of the curli fimbriae. Curli are coiled surface structures that assemble preferentially at growth temperatures below 37 degrees Celsius. Curli can bind to fibronectin.
  • 基因功能參考文獻(xiàn)：
    1. CsgA deletion mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells. PMID: 26991275
    2. A Monte Carlo study of the early steps of functional amyloid formation of bacterial biofilm, which is primarily formed by aggregates of the protein CsgA, has been presented. PMID: 26745180
    3. CsgA polymerization in vitro is preceded by the formation of thin needlelike protofibrils followed by aggregation of the amyloid fibrils. PMID: 21877724
    4. Data describe aspartic acid and glycine residues that function as "gatekeepers" to modulate CsgA polymerization efficiency and potential toxicity. PMID: 19966296
    5. At least three of five repeating units of CsgA are amyloidogenic, suggesting that covalent linkage of multiple amyloidogenic units facilitates efficient fiber formation. PMID: 17164238

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  • 亞細(xì)胞定位：
    Fimbrium. Note=Part of the curli surface structure.
  • 蛋白家族：
    CsgA/CsgB family
  • 數(shù)據(jù)庫鏈接：