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  4. Recombinant Escherichia coli O157:H7 Peptide deformylase (def)

Recombinant Escherichia coli O157:H7 Peptide deformylase (def)

In Stock
  • 中文名稱：
    Recombinant Escherichia coli O157:H7 Peptide deformylase(def)
  • 貨號：
    CSB-YP017707EOD
  • 規(guī)格：
    ￥2616
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    def
  • Uniprot No.：
  • 別名：
    def; fms; Z4657; ECs4152Peptide deformylase; PDF; EC 3.5.1.88; Polypeptide deformylase
  • 種屬：
    Escherichia coli O157:H7
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Yeast
  • 分子量：
    21.2kDa
  • 表達(dá)區(qū)域：
    2-169aa
  • 氨基酸序列
    SVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVPRAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVEKLDRLKARA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

問答及客戶評論

■ 常見問題解答
Q:

I tested this protein in a functional assay, and found that it had low-levels of activity. Apparently, metal ions are necessary for the enzymatic function of this protein, and they were curious to know what metal ions (Zn ions, Fe ions, and Ni ions), if any, were specifically added to the culture medium. Could you please advise on which ones and at what concentrations?
Also, I was wondering if you had the ability to increase the concentration of metal ions, specifically 20 mM NiCl2, throughout the expression/purification process, to try and achieve higher activity. Would this be something your lab might be able to do for the customer?
I am fine with ordering the protein again, just as long as I can confirm to them that I can provide them with higher concentrations of metal ions during the expression/purification process. I also provided the article , which explains how to increase the metal ion concentrations to achieve higher activity.
Can you please let me know if any of this would be possible?

A:
Thank you very much for your feedback and the attached article. We could satisfy your requirement and increase the concentration of metal ions, specifically 20 mM NiCl2, to achieve higher activity, according to the method in the literature. Please kindly remark these requirements when sending your PO.
As we need to reproduce this protein, the lead time for the re-order will be 15-25 working days.
We hope you could share your re-testing result with us.

靶點(diǎn)詳情

  • 功能：
    Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.
  • 蛋白家族：
    Polypeptide deformylase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ece:Z4657

    STRING: 155864.Z4657