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  4. Recombinant Escherichia coli Peptidyl-tRNA hydrolase YaeJ (yaeJ)

Recombinant Escherichia coli Peptidyl-tRNA hydrolase YaeJ (yaeJ)

  • 中文名稱：
    Recombinant Escherichia coli Peptidyl-tRNA hydrolase YaeJ(yaeJ)
  • 貨號(hào)：
    CSB-YP334759ENV
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Peptidyl-tRNA hydrolase YaeJ(yaeJ)
  • 貨號(hào)：
    CSB-EP334759ENV
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Peptidyl-tRNA hydrolase YaeJ(yaeJ)
  • 貨號(hào)：
    CSB-EP334759ENV-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Peptidyl-tRNA hydrolase YaeJ(yaeJ)
  • 貨號(hào)：
    CSB-BP334759ENV
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Escherichia coli Peptidyl-tRNA hydrolase YaeJ(yaeJ)
  • 貨號(hào)：
    CSB-MP334759ENV
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    arfB
  • Uniprot No.：
  • 別名：
    arfB; yaeJ; b0191; JW0187Peptidyl-tRNA hydrolase ArfB; PTH; EC 3.1.1.29; Alternative ribosome-rescue factor B
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-140
  • 氨基酸序列
    MIVISRHVAI PDGELEITAI RAQGAGGQHV NKTSTAIHLR FDIRASSLPE YYKERLLAAS HHLISSDGVI VIKAQEYRSQ ELNREAALAR LVAMIKELTT EKKARRPTRP TRASKERRLA SKAQKSSVKA MRGKVRSGRE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Rescues stalled ribosomes. Can hydrolyze peptidyl-tRNA on ribosomes stalled by both non-stop mRNAs and mRNAs that contain rare codon clusters or ribosomes stalled in the middle of mRNA. First identified as a complementary ribosome rescue system when the stalled ribosome cannot be rescued by the SsrA(tmRNA)-SmpB quality control system or the alternative ribosome-rescue factor A (arfA).
  • 基因功能參考文獻(xiàn)：
    1. the efficiency of the ArfA-dependent process decreases rapidly with an increase in mRNA length downstream of the A site of the ribosome whereas YaeJ function is maintained on mRNA with sufficient length. PMID: 22922063
    2. 3.2 A crystal structure of YaeJ bound to 70S ribosome in complex with the initiator tRNA(i)(fMet) and a short mRNA; structure reveals the C-terminal tail of YaeJ functions as a sensor to discriminate between stalled and actively translating ribosomes PMID: 22422986
    3. In this study, the authors identified the E. coli yaeJ gene, encoding a peptide-release factor homologue with GGQ motif, as a multicopy suppressor of the lethal phenotype of ssrA arfA double mutant. PMID: 21418110
    4. Results suggest that YaeJ functions as a ribosome-attached rescue device for stalled ribosomes. PMID: 21051357
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Prokaryotic/mitochondrial release factor family
  • 數(shù)據(jù)庫(kù)鏈接：