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  4. Recombinant Glycine max 2S seed storage albumin protein

Recombinant Glycine max 2S seed storage albumin protein

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  • 中文名稱：
    Recombinant Glycine max 2S seed storage albumin protein
  • 貨號：
    CSB-YP324829GGV
  • 規(guī)格：
    ￥2616
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    2S albumin; GM2S-1; Napin-type 2S albumin 3) [Cleaved into: 2S albumin small chain; Aspartic acid-rich peptide; Lunasin); 2S albumin large chain; 8 kDa methionine-rich protein; 8 kDa MRP)]
  • 種屬：
    Glycine max (Soybean) (Glycine hispida)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Yeast
  • 分子量：
    18.2kDa
  • 表達區(qū)域：
    22-158aa
  • 氨基酸序列
    SKWQHQQDSCRKQLQGVNLTPCEKHIMEKIQGRGDDDDDDDDDNHILRTMRGRINYIRRNEGKDEDEEEEGHMQKCCTEMSELRSPKCQCKALQKIMENQSEELEEKQKKKMEKELINLATMCRFGPMIQCDLSSDD
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The production of this Recombinant Glycine max Musk protein began at the genetic level, where the coding sequence for the Musk protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (Yeast) so that the cells could be cultured and expressed the desired 2S albumin protein. And we finally got the recombinant 2S albumin protein with the purity of 90%+ determined by SDS-PAGE.

    2S albumins, defined on the basis of their sedimentation coefficient, are a major group of seed storage proteins widely distributed in both mono- and di-cotyledonous plants. As storage proteins, they are deposited in protein bodies of developing seeds and are utilized by the plant as a source of nutrients (amino acids and carbon skeletons) during subsequent germination and seedling growth. Recent findings have demonstrated that 2S albumins can also play a protective role in plants as defensive weapons against fungal attack. In addition to their physiological role in plants, these small globular proteins are becoming of increasing interest in nutritional and clinical studies. The amino acid composition of 2S albumin proteins from many plant species has revealed their high content of sulphur-containing amino acids.

     

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    This is a 2S seed storage protein.; binds to mammalian chromatin, preventing the normal formation of the kinetochore complex in the centromere and leading to the disruption of mitosis.
  • 基因功能參考文獻：
    1. A potential strategy for recombinant production of bioactive lunasin in industry: production of anti-inflammatory agent by cloning a tandem repeat of Lunasin in Pichia pastoris. PMID: 29506820
    2. lunasin treatment could reverse the effects of ox-LDL on the expression of eNOS and PAI-1, the direct target genes of KLF2. Mechanistically, it was proven that the MEK5/ERK5 pathway mediates the effects of lunasin on KLF2 expression. PMID: 29096169
    3. lunasin will have promising potential as a novel nutritional supplement or drug candidate for RA due to its potency of suppressing synovial cell proliferation PMID: 25692134
  • 蛋白家族：
    2S seed storage albumins family
  • 組織特異性：
    Expressed in cotyledons (Ref.2). Maximal expression in parenchyma cells undergoing DNA endoreduplication and cell expansion but not in actively dividing cells of the cotyledon.
  • 數(shù)據(jù)庫鏈接：

    KEGG: gmx:548076

    STRING: 3847.GLYMA13G36400.1

    UniGene: Gma.32309