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  4. Recombinant Helicobacter pylori Urease subunit beta (ureB)

Recombinant Helicobacter pylori Urease subunit beta (ureB)

In Stock
  • 中文名稱：
    幽門螺旋桿菌ureB重組蛋白
  • 貨號(hào)：
    CSB-EP300910HUV
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ureB
  • Uniprot No.：
  • 別名：
    HP_0072; hpuB; URE1_HELPY; Urea amidohydrolase subunit beta; Urease subunit beta; ureB
  • 種屬：
    Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    E.coli
  • 分子量：
    65.7kDa
  • 表達(dá)區(qū)域：
    1-569aa
  • 氨基酸序列
    MKKISRKEYVSMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSNNPSKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEALAGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKWMLRAAEEYSMNLGFLAKGNASNDASLADQIEAGAIGFKIHEDWGTTPSAINHALDVADKYDVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTFHTEGAGGGHAPDIIKVAGEHNILPASTNPTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSSDSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISEYVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAHHGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCRNITKKDMQFNDTTAHIEVNPETYHVFVDGKEVTSKPANKVSLAQLFSIF
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The recombinant H. pylori ureB protein is encoded by the gene of ureB (1-569aa). The gene of ureB was cloned in a system (E.coli) that supported the expression of ureB and translation of messenger RNA. Modification of ureB by recombinant DNA technology could lead to the expression of the target protein. The protein was fused with N-terminal 6xHis tag in the production. The purity is 90% determined by SDS-PAGE.

    UreB is a protein coding gene that encodes Urease subunit beta. According to some studies, UreB may have the following features.
    The ureA and ureB genes encoding H. pylori urease are transcribed and translated in E. coli, and these genes alone are sufficient to synthesize and assemble the bulk-sized enzyme. The basic properties of H. pylori urease activity on colonization indicate that H. pylori urease is an excellent reporter of gene expression in H. pylori. Recombinant L. lactis expressing UreB-IL-2 has the potential to be used as an edible vaccine for the control of Helicobacter pylori infection. UreB transgenic carrots have the potential to be used as an edible vaccine for the control of Helicobacter pylori. UreB enhances the stability of UreC against proteolysis.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.
  • 基因功能參考文獻(xiàn)：
    1. Helicobacter urease-induced activation of the TLR2/NLRP3/IL-18 axis protects against asthma. PMID: 26214524
  • 亞細(xì)胞定位：
    Cytoplasm. Note=Also associates with the outer membrane upon autolysis of neighboring bacteria.
  • 蛋白家族：
    Metallo-dependent hydrolases superfamily, Urease alpha subunit family
  • 數(shù)據(jù)庫(kù)鏈接：