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  4. Recombinant Hepatitis E virus genotype 1 Secreted protein ORF2 (ORF2), partial

Recombinant Hepatitis E virus genotype 1 Secreted protein ORF2 (ORF2), partial

  • 中文名稱：
    Recombinant Hepatitis E virus genotype 1 Secreted protein ORF2(ORF2),partial
  • 貨號：
    CSB-EP727851HVZ
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ORF2
  • Uniprot No.：
  • 別名：
    ORF2Secreted protein ORF2; Protein ORF2; pORF2
  • 種屬：
    Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    84.8kDa
  • 表達區(qū)域：
    20-660aa
  • 氨基酸序列
    PPPGQPSGRRRGRRSGGSGGGFWGDRVDSQPFAIPYIHPTNPFAPNVTAAAGAGPRVRQPVRPLGSAWRDQAQRPAAASRRRPTTAGAAPLTAVAPAHDTPPVPDVDSRGAILRRQYNLSTSPLTSSVATGTNLVLYAAPLSPLLPLQDGTNTHIMATEASNYAQYRVARATIRYRPLVPNAVGGYAISISFWPQTTPTPTSVDMNSITSTDVRILVQPGIASELVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCIHGSPVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYSSTARHRLRRGADGTAELTTTAATRFMKDLYFTSTNGVGEIGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSRPVVSANGEPTVKLYTSVENAQQDKGIAIPNDIDLGESRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTIQQYSKIFFVLPLRGKLSFWEAGTTRPGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLGPHSALALLEDTLDYPARAHTFDDFCPECRPLGLQGCAFQSTVAELQRLKMKVGKTREL
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    To prepare this Recombinant HEV-1 ORF2 protein, the recombinant DNA was required, which was generated by fusing the ORF2 gene with N-terminal 6xHis-SUMO tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this ORF2 protein production. After purification, a premium ORF2 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 90%+.

    ORF2 is a protein encoding gene that provides an instruction in making a protein named secreted protein ORF2 or protein ORF2. In Hepatitis E virus (HEV), the protein encoded by ORF2 gene has two isoforms, secreted protein ORF2 and capsid protein ORF2. The sequence difference between isoform 2 and the isoform 1 is lack of the 1-15 amino acid sequence. The difference between the sequence directly determines their different functions. Isoform 1 can inhibit host antibody-mediated neutralization without blocking viral cell entry. Isoform 2 is the major virion component to form the viral capsid. ORF2 from HEV plays multiple functions in both viral replication and pathogenesis.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays a role in the inhibition of host antibody-mediated neutralization without blocking viral cell entry.; Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation. Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes.
  • 亞細胞定位：
    [Isoform Secreted protein ORF2]: Secreted.; [Isoform Capsid protein ORF2]: Virion. Host cytoplasm. Host endoplasmic reticulum. Host Golgi apparatus. Host cell surface.
  • 蛋白家族：
    Hepevirus capsid protein family