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  4. Recombinant Human Ribonuclease H1 (RNASEH1)

Recombinant Human Ribonuclease H1 (RNASEH1)

In Stock
  • 中文名稱：
    人RNASEH1重組蛋白
  • 貨號(hào)：
    CSB-EP019801HU
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019801HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RNASEH1.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019801HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RNASEH1.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    RNASEH1; RNH1; Ribonuclease H1; RNase H1; EC 3.1.26.4; Ribonuclease H type II
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    E.coli
  • 分子量：
    37.6 kDa
  • 表達(dá)區(qū)域：
    1-286aa
  • 氨基酸序列
    MSWLLFLAHRVALAALPCRRGSRGFGMFYAVRRGRKTGVFLTWNECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLREPLDGDGHESAEPYAKHMKPSVEPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer,50% glycerol
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
        核糖核酸酶H1（Ribonuclease H1，RNASEH1）是一種核酸酶，主要參與DNA合成中的RNA剪切。RNASEH1的主要功能是在DNA合成時(shí)去除RNA片段，確保新合成的DNA鏈的完整性。這一過(guò)程對(duì)于維護(hù)基因組的穩(wěn)定性至關(guān)重要，因?yàn)樵贒NA合成期間，RNA片段會(huì)暫時(shí)嵌入新合成的DNA鏈。
        華美生物提供的重組人RNASEH1蛋白是由大腸桿菌表達(dá)系統(tǒng)表達(dá)而成的全長(zhǎng)重組蛋白。。該產(chǎn)品可用于廣泛的分子生物學(xué)實(shí)驗(yàn)中，特別是在研究DNA合成、RNA剪切和核酸互動(dòng)等方面。
        RNASEH1在DNA修復(fù)和維護(hù)基因組完整性中起著重要作用。它的缺陷與一些疾病，如Ataxia Telangiectasia-like Disorder（ATLD）等，有關(guān)。因此，研究RNASEH1的結(jié)構(gòu)和功能有助于深入了解DNA代謝的調(diào)控機(jī)制，同時(shí)有助于解析與該酶相關(guān)的遺傳性疾病的發(fā)病機(jī)制。華美生物重組RNASEH1蛋白的提供將為科學(xué)家在DNA修復(fù)、基因組穩(wěn)定性、疾病發(fā)病機(jī)制等領(lǐng)域的研究提供有力的支持，促進(jìn)相關(guān)領(lǐng)域的科學(xué)進(jìn)展。
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.
  • 基因功能參考文獻(xiàn)：
    1. RNASEH1 gene variants associate with susceptibility/protection to T1 Diabetes in Colombia. PMID: 29204916
    2. Studies indicate that ribonuclease H1 is essential for mitochondrial DNA replication. PMID: 27402764
    3. Data show that the catalytic domains of E. coli and human RNase H have nearly identical sequence preferences, which correlate with the efficiency of RNase H-recruiting antisense oligonucleotides. PMID: 29126318
    4. Data suggest that ribonuclease H1 (RNASEH1) plays important role in replication fork movement by resolving R-loops (RNA-DNA hybrids); RNASEH1 depletion results in accumulation of RNA-DNA hybrids, slowing of replication forks, and increased DNA damage; RNASEH1 appears to contribute to genome stability and preserves telomere integrity. PMID: 28717002
    5. RPA is a sensor of R loops and a regulator of RNaseH1, extending the versatile role of RPA in suppression of genomic instability. PMID: 28257700
    6. RNaseH1 maintains regulated levels of telomeric RNA-DNA hybrids at ALT telomeres to trigger homologous recombination without compromising telomere integrity too severely PMID: 25330849
    7. found that the 3' fragments of target pre-mRNA generated by ASO were almost completely degraded from their 5' ends by nuclear XRN2 after RNase H1-mediated cleavage PMID: 26159921
    8. Altered RNaseH1 has a reduced capability to remove the RNA from RNA-DNA hybrids leading to impaired mtDNA replication and adult-onset mitochondrial encephalomyopathy. PMID: 26094573
    9. RNase H1 and protein P32 are involved in mitochondrial pre-rRNA processing PMID: 23990920
    10. data implicate the H264 side chain in phosphodiester hydrolysis as well as in product release, and are consistent with a proposed model in which the RNAse H1 H264 side chain interacts with a divalent metal ion to support catalysis PMID: 23078533
    11. On the basis of its nuclear magnetic resonance (NMR) nucleic acid structure, a boranophosphonate-modified, fully R(P) BH(3) DNA/RNA hybrid is predicted not to be a substrate for RNase H1. PMID: 21443203
    12. Observational study of gene-disease association. (HuGE Navigator) PMID: 20877624
    13. The cysteine residues responsible for the redox-dependent activity of RNase H1 were determined by site-directed mutagenesis to involve Cys(147) and Cys(148), producing an inactive enzyme conformation by disulfide bond formation. PMID: 12473655
    14. Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate PMID: 14506260
    15. in human cells RNase H1 is responsible for most of the activity of DNA-like antisense drugs PMID: 14960586
    16. analysis of catalytic site of human RNase H1 for heteroduplex substrate catalysis PMID: 15205459
    17. method for enhancing the human RNase H1 activity of chimeric antisense oligonucleotides PMID: 17028157
    18. THE role substrate structure plays in directing human RNase H1 activity as well as the design of effective antisense oligodeoxyribonucleotides. PMID: 17028158
    19. Report crystal structures of RNase H1 in complex with RNA/DNA hybrids. PMID: 17964265
    20. Characterization of full-length enzymes with defective hybrid binding domain indicates that this domain dramatically enhances both the specific activity and processivity of RNase H1. PMID: 18337749

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  • 相關(guān)疾?。?/div>
    Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2 (PEOB2)
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    RNase H family
  • 組織特異性：
    Ubiquitous.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 18466

    OMIM: 604123

    KEGG: hsa:246243

    STRING: 9606.ENSP00000313350

    UniGene: Hs.568006