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  4. Recombinant Mouse Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 (Ndst2), partial

Recombinant Mouse Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 (Ndst2), partial

  • 中文名稱(chēng)：
    小鼠Ndst2重組蛋白
  • 貨號(hào)：
    CSB-YP015615MO
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    小鼠Ndst2重組蛋白
  • 貨號(hào)：
    CSB-EP015615MO-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    小鼠Ndst2重組蛋白
  • 貨號(hào)：
    CSB-BP015615MO
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    小鼠Ndst2重組蛋白
  • 貨號(hào)：
    CSB-MP015615MO
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ndst2
  • Uniprot No.：
  • 別名：
    Ndst2; Hsst2; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2; EC 2.8.2.8; Glucosaminyl N-deacetylase/N-sulfotransferase 2; NDST-2; Mndns; N-heparan sulfate sulfotransferase 2; N-HSST 2) [Includes: Heparan sulfate N-deacetylase 2; EC 3.-.-.-); Heparan sulfate N-sulfotransferase 2; EC 2.8.2.-)]
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Required for the exosomal release of SDCBP, CD63 and syndecan.
  • 基因功能參考文獻(xiàn)：
    1. Size estimation of heparan sulfate polysaccharide chains indicated that increased chain lengths in NDST2-overexpressing cells alone could explain the increased heparan sulfate content. A model is discussed where NDST2-specific substrate modification stimulates elongation resulting in increased heparan sulfate chain length. PMID: 27387504
    2. embryonic stem cells lacking both NDST1 and NDST2, expressing a very low sulfated heparan sulfate, can take the initial step toward differentiation into all three germ layers PMID: 22298785
    3. although NDST-2(-/-) mice demonstrate an altered response in interstitial fulid pressure after mast cell activation, there was no similar alteration after neurogenic inflammation PMID: 14604857
    4. NDST-2 does not contribute significantly to tissue-specific Heparan Sulfate structures PMID: 15292174
    5. NDST1 and NDST2 have roles in N-sulfation but not in 6-O-sulfation of heparan sulfate PMID: 15319440
    6. analysis of liver NDST1 and NDST2 biosynthesis PMID: 16984905
    7. expression of N-deacetylase/N-sulfotransferase (NDST)-2, a key enzyme in mast cell maturation PMID: 19915053

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  • 亞細(xì)胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Sulfotransferase 1 family, NDST subfamily
  • 組織特異性：
    Widely expressed in adult and throughout development.
  • 數(shù)據(jù)庫(kù)鏈接：