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  4. Recombinant Mouse Epidermal growth factor-like protein 8 (Egfl8)

Recombinant Mouse Epidermal growth factor-like protein 8 (Egfl8)

In Stock
  • 中文名稱：
    小鼠Egfl8重組蛋白
  • 貨號：
    CSB-EP753592MO
  • 規(guī)格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Egfl8
  • Uniprot No.：
  • 別名：
    Egfl8; Ng3Epidermal growth factor-like protein 8; EGF-like protein 8
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    49.0 kDa
  • 表達區(qū)域：
    29-293aa
  • 氨基酸序列
    GSFKESLGVCSKQTLLVPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKKPHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCLNTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAELRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEERLGACACEDNSLGPSLRG
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer,50% glycerol
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    This recombinant Mouse Egfl8?protein is typically achieved by the manipulation of Egfl8?gene expression in E.coli?cells so that it expresses large amounts of a recombinant Egfl8 gene. In order to get enough amount of the Egfl8?protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the Egfl8 protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.

    Egfl8 plays negative regulatory roles in mouse thymic epithelial cells (TECs) and thymocytes. It has shown that Egfl8 is downregulated in gastric cancer (GC) and is closely associated with high tumor-node-metastasis stage and dismal prognosis in gastric and colorectal cancer. In addition to participating in thymopoiesis, Egfl8 is also involved in cell migration and invasion by regulating Notch signaling. Egfl8 also can act as a neuritogen and rewire cellular signaling by activating kinases involved in neurogenesis.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 基因功能參考文獻：
    1. Mouse EGFL8 encodes a protein of 293 amino acids with two EGFL domains, an Emilin-like domain and a Ca(2+)-binding EGFL domain, which has a molecular mass of 32 kDa. PMID: 26096343
    2. EGFL8 inhibited thymocyte proliferation and induced thymocyte apoptosis. PMID: 23877103
    3. these data show that EGFL8 exerts inhibitory effects on thymic epithelial cells and thymocytes, suggesting that EGFL8 acts as a negative regulatory molecule in the development of T cells in the mouse thymus. PMID: 22828506
  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Ubiquitously expressed in brain, kidney, thymus and lung.
  • 數(shù)據(jù)庫鏈接：