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  4. Recombinant Mouse Factor in the germline alpha (Figla)

Recombinant Mouse Factor in the germline alpha (Figla)

  • 中文名稱：
    小鼠Figla重組蛋白
  • 貨號：
    CSB-YP008675MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Figla重組蛋白
  • 貨號：
    CSB-EP008675MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Figla重組蛋白
  • 貨號：
    CSB-EP008675MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Figla重組蛋白
  • 貨號：
    CSB-BP008675MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Figla重組蛋白
  • 貨號：
    CSB-MP008675MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Figla
  • Uniprot No.：
  • 別名：
    FiglaFactor in the germline alpha; FIGalpha; Transcription factor FIGa
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-194
  • 氨基酸序列
    MDTAPASPEP FLVTPQAEVL EELIQAQMGP LPRLAAICRL KRLPSGGYST TDDLHLVLER RRVANAKERE RIKNLNRGFA KLKALVPFLP QSRKPSKVDI LKGATEYIQI LGCVLEEAKV SEKQSPEEQT HSGRPSDPHV SSTRELLGNA TQPTSCASGL KKEEEGPWAY AGHSEPLYSY HQSTVPETRS YFTH
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Germ-line specific transcription factor implicated in postnatal oocyte-specific gene expression. Plays a key regulatory role in the expression of multiple oocyte-specific genes, including those that initiate folliculogenesis and those that encode the zona pellucida (ZP1, ZP2 and ZP3) required for fertilization and early embryonic survival. Essential for oocytes to survive and form primordial follicles. The persistence of FIGLA in adult females suggests that it may regulate additional pathways that are essential for normal ovarian development. Binds to the E-box (5'-CANNTG-3') of the ZPs (ZP1, ZP2, ZP3) promoters.
  • 基因功能參考文獻：
    1. GFP positive cells showed the expression of germ cell markers Vasa, meiotic specific gene Stra8, Scp3, oocyte markers Gdf9, Zp3 and Figla, indicating that this method could be used for the purification and selection of female germ cells PMID: 22213070
    2. Data suggest that a dynamic DNA CpG methylation in Sohlh2 and Figlalpha promoters, but not in the intron and exon sequences, is linked to regulation of gene expression, even though CpG islands are also present in their introns or exons. PMID: 21427160
    3. physiological expression of Figla plays a critical dual role in activation of oocyte-associated genes and repression of sperm-associated genes during normal postnatal oogenesis PMID: 20479125
    4. FIGLA is a central regulator of oocyte-specific genes that play roles in folliculogenesis, fertilization and early development. PMID: 17567914
  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    Expressed only in the oocytes within the ovary and at lower level in the testis. Found in the resting oocytes of the primordial follicle cells, at the periphery of the ovary and in the hilar region. Also detected in growing oocytes, but at lower levels.
  • 數(shù)據(jù)庫鏈接：