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  4. Recombinant Mouse Leucine-rich repeat serine/threonine-protein kinase 2 (Lrrk2), partial

Recombinant Mouse Leucine-rich repeat serine/threonine-protein kinase 2 (Lrrk2), partial

  • 中文名稱：
    小鼠Lrrk2重組蛋白
  • 貨號：
    CSB-YP716267MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Lrrk2重組蛋白
  • 貨號：
    CSB-EP716267MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Lrrk2重組蛋白
  • 貨號：
    CSB-EP716267MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Lrrk2重組蛋白
  • 貨號：
    CSB-BP716267MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Lrrk2重組蛋白
  • 貨號：
    CSB-MP716267MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Lrrk2; Leucine-rich repeat serine/threonine-protein kinase 2; EC 2.7.11.1; EC 3.6.5.-
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, autophagy, and vesicle trafficking. Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation. Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43. Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A. Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A. Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose-6-phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Phosphorylates PRDX3. By phosphorylating APP on 'Thr-743', which promotes the production and the nuclear translocation of the APP intracellular domain (AICD), regulates dopaminergic neuron apoptosis. Independent of its kinase activity, inhibits the proteosomal degradation of MAPT, thus promoting MAPT oligomerization and secretion. In addition, has GTPase activity via its Roc domain which regulates LRKK2 kinase activity.
  • 基因功能參考文獻(xiàn)：
    1. In conclusion, Lrrk2 is a powerful regulator of Na(+)/K(+) -ATPase expression and activity in dendritic cells. PMID: 28120865
    2. These results implicate the stress-responsive machinery composed of Rab7L1, LRRK2, phosphorylated Rab8/10, and their downstream effectors in the maintenance of lysosomal homeostasis. PMID: 30209220
    3. Genetic susceptibility in the form of LRRK2 mutation conferred a significant vulnerability to long-term environmental toxicity as the mice aged over half their lifespan, involving reduced striatal mitochondrial Complex-I levels. PMID: 28098219
    4. LRRK2-induced dopaminergic and norepinephrine neurodegeneration is kinase-dependent and can occur in a cell-autonomous manner. PMID: 29386392
    5. Findings suggest the importance of murine LRRK2 G2019S variant in hippocampal and striatal neurotransmission, but that the transgene didn't appear to be involved in functional behavioral and stress-like hormonal and cytokine changes provoked by inflammatory insults. PMID: 28893563
    6. LRRK2 functions together with a second Parkinson's disease-associated gene, RAB7L1, within an evolutionarily conserved genetic module in diverse cellular contexts. PMID: 27424887
    7. Slowing of dopamine transients is observed with age in littermates, suggesting premature ageing of dopamine synapses in LRRK2 G2019S knock-in mice. PMID: 28930069
    8. This study demonstrated that at old age the homozygous LRRK2 R1441C animals exhibit clear phenotypes related to the prodromal phase of PD such as impairments in fine motor tasks, gait, and olfaction. PMID: 28576705
    9. inhibition of LRRK2 could not only decrease the expression of inflammatory cytokines, but also recover autophagic function of microglia. PMID: 29408508
    10. Study showed that LRRK2 phosphorylation is required for LRRK2 to bind to Fbxl18, and that this interaction is important for regulating the amount of LRRK2 via ubiquitination of LRRK2 by the SCFFbxl18 ubiquitin ligase and subsequent proteasomal degradation of LRRK2. We also show that LRRK2 can induce caspase 8 mediated cell death and that the degradation of LRRK2 mediated by Fbxl18 can prevent LRRK2-mediated toxicity. PMID: 27890708
    11. Loss of LRRK2 causes increased canonical Wnt activity in vitro and in vivo PMID: 28103901
    12. LRRK2 disruptes aggresome formation for autophagy. PMID: 27364102
    13. findings revealed an essential role of LRRK in the survival of DA neurons and in the regulation of the autophagy-lysosomal pathway in the aging brain. PMID: 29056298
    14. PD mutation LRRK2 G2019S impairs synaptic vesicle endocytosis in ventral midbrain dopaminergic neurons. PMID: 29054882
    15. Parkinson's disease-linked LRRK2 mutations increased dendritic and mitochondrial calcium uptake in cortical neurons. PMID: 29038245
    16. Study found that the transcripts of the Lrrk2 gene in mice, which encodes a multifunctional protein with kinase and GTPase activities, is upregulated only in Inflexible Drinkers suggesting, for the first time, that the Lrrk2 pathway plays a major role in the compulsive ethanol intake behaviour of addicted subjects. PMID: 27411784
    17. promotes activation of NLRC4 inflammasome during Salmonella Typhimurium infection PMID: 28821568
    18. The Parkinson's disease-associated LRRK2 mutant G2019S impairs DRD1 internalization, leading to an alteration in signal transduction. The mutant forms of LRRK2 also affect receptor turnover by decreasing the rate of DRD2 trafficking from the Golgi complex to the cell membrane. PMID: 28582422
    19. In addition to disease modeling, leucine-rich repeat kinase 2 (LRRK2) konckin (KI) mice represent a valuable resource for pharmacological studies. PMID: 28202664
    20. These results of this study demonstrate that alpha-synuclein inclusion formation in neurons can be blocked and that novel therapeutic compounds targeting this process by inhibiting LRRK2 kinase activity may slow progression of PD-associated pathology. PMID: 27413152
    21. The findings of this study indicated that the G2019S mutation of LRRK2 imparts a gain-of-abnormal function to striatal spiny projection neurons activity and morphology during a stage of development when activity can permanently modify circuit structure and function. PMID: 27383589
    22. LRRK2 is expressed in enteric neurons and related to alterations of neuronal peptides and IgA. PMID: 28168579
    23. this study reveals that LRRK2 is a new positive regulator of Rip2 and promotes inflammatory cytokine induction through the Nod1/2-Rip2 pathway. PMID: 27830463
    24. an altered sphingolipid composition in Lrrk2(-/-) mouse brains, is reported. PMID: 27539321
    25. findings reveal a novel function of LRRK2 in regulating EPO expression and imply a potentially novel relationship between PD genes and hematopoiesis. PMID: 27872856
    26. In the present study demonstrated that the up-regulation of HOTAIR and LRRK2 in the midbrain of PD mice induced by MPTP. PMID: 26979073
    27. that mutant LRRK2 may impair synaptic vesicle dynamics via aberrant phosphorylation of N-ethylmaleimide sensitive fusion PMID: 26758690
    28. 14-3-3theta can regulate LRRK2 and reduce the toxicity of mutant LRRK2 through a reduction of kinase activity. PMID: 26546614
    29. LRRK2 has a role in microglia activation and sustainment of neuroinflammation. PMID: 26646749
    30. p21-activated kinase 6 (PAK6) as a novel interactor of leucine-rich repeat kinase 2 (LRRK2), a kinase involved in Parkinson's disease PMID: 26375402
    31. The data implicate gain of LRRK2 function in the pathogenesis of Parkinson disease PMID: 26282470
    32. LRRK2 regulates lysosome size, number and function in primary astrocytes and results show that expression of Parkinson's disease associated LRRK2 variants results in enlarged lysosomes PMID: 26251043
    33. Present results suggest that LRRK2 may play novel roles in both the immune system and the pathogenesis of various diseases. PMID: 26943952
    34. Selective expression of Parkinson's disease-related Leucine-rich repeat kinase 2 G2019S missense mutation in midbrain dopaminergic neurons impairs dopamine release and dopaminergic gene expression PMID: 26123485
    35. In the substantia nigra of LRRK2 transgenic mice, mRNA of three genes that promote cell death was upregulated, while the mRNA expression of seven genes that contribute to neurogenesis/neuroprotection was downregulated. PMID: 26363496
    36. G2019S mutation carrying LRRK2 microglia show retarded ADP-induced motility and delayed isolation of injury. LRRK2 knockdown microglia are highly motile compared with control cells. Lrrk2 phosphorylates and inhibits focal adhesion kinase. PMID: 26365310
    37. The Lrrk2 (-/-) mice developed lower levels of experimental autoimmune uveitis, delayed type hypersensitivity responses and cytokine production by lymphocytes than did their WT controls. PMID: 26067490
    38. The results indicate that LRRK2 kinase activity has an important role in the regulation of clathrin-mediated endocytosis of synaptic vesicles and subsequent neurotransmission at the synapse. PMID: 25501810
    39. Study demonstrates that mice expressing just one or two copies of mutant G2019S LRRK2 in their own genome have significantly elevated kinase activity and progressive dopaminergic alterations PMID: 25836420
    40. Membrane-associated LRRK2 co-localized to autophagosome membranes following either TLR4 stimulation or mTOR inhibition with rapamycin. PMID: 24682598
    41. Results suggest that LRRK2 alters actin dynamics and F-actin structure both in brain neurons and skin fibroblasts, indicate that LRRK2-dependent changes in the cytoskeleton might have functional consequences on postsynaptic NMDA receptor localization PMID: 25301747
    42. LRRK2 overexpression produces exploratory hypoactivity, recognition memory impairments, altered glutamatergic short-term plasticity and reduced dopamine tone. PMID: 25343991
    43. LRRK2 facilitates tau phosphorylation indirectly by recruiting tau or cdk5 rather than by directly phosphorylating tau PMID: 26268594
    44. we find that overexpression of LRRK2-G2019S increases basal synaptic efficiency through a postsynaptic mechanism, and disrupts long-term depression in the hippocampus PMID: 26269629
    45. Calyculin A treatment prevents inhibitor and PD mutant induced dephosphorylation and reverts LRRK2 to a lesser ubiquitinated species, thus directly implicating phosphatase activity in LRRK2 ubiquitination PMID: 25939886
    46. In vitro kinase assays and metabolic-labeling experiments in living cells confirmed MARK1 as an LRRK2 substrate. Moreover, we also showed that LRRK2 and MARK1 are interacting in eukaryotic cells. PMID: 25854701
    47. conclude that pathologic activation of LRRK2 regulates a significant component of the neuroinflammation associated with Hiv associatedneurocognitive disorder. PMID: 25834052
    48. Study evaluates LRRK2 expression in mouse and rat brain to aid in determining the normal function of LRRK2 and in selecting rodent models for studying pathogenic LRRK2 mutations PMID: 24633735
    49. Results indicate that mutant LRRK2 does not affect PGRN mRNA expression or intracellular PGRN protein levels/PGRN degradation in cell culture PMID: 24652679
    50. these results define a pathway for LRRK2 extracellular release, clarify one function of the LRRK2 14-3-3 interaction and provide a foundation for utilization of LRRK2 as a biomarker in clinical trials. PMID: 23886663

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  • 亞細(xì)胞定位：
    Cytoplasmic vesicle. Perikaryon. Cell projection, axon. Cell projection, dendrite. Golgi apparatus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family
  • 組織特異性：
    Expressed in the brain (at protein level). Detected throughout the adult brain. Expressed in deep cerebral cortex layers, superficial cingulate cortex layers, the piriform cortex, hippocampal formation, caudate putamen, substantia nigra, the basolateral a
  • 數(shù)據(jù)庫鏈接：