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  4. Recombinant Mesomycoplasma hyopneumoniae 46 kDa surface antigen (p46)

Recombinant Mesomycoplasma hyopneumoniae 46 kDa surface antigen (p46)

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  • 中文名稱：
    Recombinant Mesomycoplasma hyopneumoniae 46 kDa surface antigen(p46)
  • 貨號：
    CSB-EP313811MWKa2
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    p46
  • Uniprot No.：
  • 別名：
    p46; mhp511; 46 kDa surface antigen; p46
  • 種屬：
    Mycoplasma hyopneumoniae (strain 232)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    58.5kDa
  • 表達(dá)區(qū)域：
    28-416aa
  • 氨基酸序列
    CGQTESGSTSDSKPQAETLKHKVSNDSIRIALTDPDNPRWISAQKDIISYVDETEAATSTITKNQDAQNNWLTQQANLSPAPKGFIIAPENGSGVGTAVNTIADKGIPIVAYDRLITGSDKYDWYVSFDNEKVGELQGLSLAAGLLGKEDGAFDSIDQMNEYLKSHMPQETISFYTIAGSQDDNNSQYFYNGAMKVLKELMKNSQNKIIDLSPEGENAVYVPGWNYGTAGQRIQSFLTINKDPAGGNKIKAVGSKPASIFKGFLAPNDGMAEQAITKLKLEGFDTQKIFVTGQDYNDKAKTFIKDGDQNMTIYKPDKVLGKVAVEVLRVLIAKKNKASRSEVENELKAKLPNISFKYDNQTYKVQGKNINTILVSPVIVTKANVDNPDA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The production of this Recombinant M.hyopneumoniae 232 mutT protein began at the genetic level, where the coding sequence for the mutT protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (E.coli) so that the cells could be cultured and expressed the desired p46 protein. And we finally got the recombinant p46 protein with the purity of 90%+ determined by SDS-PAGE.

    The crystal structure of P46, one of the main surface-antigen proteins, from M. hyopneumoniae is presented and shows N- and C-terminal α/β domains connected by a hinge. P46 has been identified as a novel natural killer cell–specific surface molecule that mediates cell activation. Beyond this, there are many findings that determined the various funcstions of P46 in cells. For example, it has been found that inactivation of runx3/p46 promotes cutaneous t-cell lymphoma; endomembrane targeting of human oas1 p46 augments antiviral activity; the cellular localization of the p42 and p46 oligoadenylate synthetase 1 isoforms and their impact on mitochondrial respiration; overexpression of the p46 (t1) translocase component of the glucose-6-phosphatase complex in hepatocytes impairs glycogen accumulation via hydrolysis of glucose 1-phosphate; enhanced expression of p46 shc in the nucleus and p52 shc in the cytoplasm of human gastric cancer.

     

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mhy:mhp511