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  4. Recombinant Phorbol ester/diacylglycerol-binding protein unc-13 (unc-13), partial

Recombinant Phorbol ester/diacylglycerol-binding protein unc-13 (unc-13), partial

  • 中文名稱：
    秀麗隱桿線蟲unc-13重組蛋白
  • 貨號：
    CSB-YP338863CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲unc-13重組蛋白
  • 貨號：
    CSB-EP338863CXY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲unc-13重組蛋白
  • 貨號：
    CSB-EP338863CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲unc-13重組蛋白
  • 貨號：
    CSB-BP338863CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲unc-13重組蛋白
  • 貨號：
    CSB-MP338863CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    unc-13
  • Uniprot No.：
  • 別名：
    unc-13; ZK524.2; Phorbol ester/diacylglycerol-binding protein unc-13; Uncoordinated protein 13
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    May form part of a signal transduction pathway, transducing the signal from diacylglycerol to effector functions. One such function could be the release of neurotransmitter from neurons. Probably by regulating neuronal transmission downstream of lin-3 and receptor lin-23 and phospholipase plc-3 and upstream of innexin unc-7 and egl-4/PKG in ALA neurons, involved in the decrease in pharyngeal pumping during the quiescent state that precedes each larval molt,.
  • 基因功能參考文獻：
    1. C1-C2B exerts a basal inhibition on Munc13 in the primed state, permitting calcium- and lipid-dependent control of C1-C2B to modulate synaptic strength PMID: 28772122
    2. Synaptic UNC13A protein variant causes increased neurotransmission and dyskinetic movement disorder PMID: 28192369
    3. results suggest that UNC-13L, UNC-13S, and Tomosyn form a molecular code that dictates the timing of neurotransmitter release PMID: 23951547
    4. data reveal multiple roles of UNC-13 C2A domain, and suggest that spontaneous release and the fast phase of evoked release may involve a common pool of SVs at the active zone PMID: 24220508
    5. The regulation of aging by unc-13 and sbt-1 is temperature-dependent. And the alterations in reproduction capability and stress response may be associated with the formation of this temperature-dependent property PMID: 19927169
    6. UNC-13 may regulate multiple steps of the synaptic-vesicle cycle, and its interaction with syntaxin is required for synaptic transmission. PMID: 16271476
    7. Even when inhibited synaptic vesicle priming is restored, synaptic transmission remains defective in unc-13 mutants, suggesting that UNC-13 is also required for other aspects of secretion. PMID: 16880125
    8. This study investigates a role for UNC-13 in RNAi through analysis of RNAi penetrance in unc-13 and eri-1 mutant strains. UNC-13 functions in conjunction with ERI-1 in RNAi. PMID: 17276405
    9. Calcium activated protein for secretion (CAPS)/UNC-31 and UNC-13 serve parallel and dedicated roles in dense-core vesicle and synaptic vesicle exocytosis, respectively, in the C. elegans nervous system. PMID: 17553987
    10. Results support a model for docking in which UNC-13 converts syntaxin from the closed to the open state, and open syntaxin acts directly in docking vesicles to the plasma membrane. PMID: 17645391

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  • 蛋白家族：
    Unc-13 family
  • 數(shù)據(jù)庫鏈接：

    STRING: 6239.ZK524.2d

    UniGene: Cel.16847