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  4. Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog, partial

Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog, partial

  • 中文名稱：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 貨號(hào)：
    CSB-YP728974PYX
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 貨號(hào)：
    CSB-EP728974PYX
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 貨號(hào)：
    CSB-EP728974PYX-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 貨號(hào)：
    CSB-BP728974PYX
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 貨號(hào)：
    CSB-MP728974PYX
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CCAR2
  • Uniprot No.：
  • 別名：
    CCAR2Cell cycle and apoptosis regulator protein 2; Cell division cycle and apoptosis regulator protein 2
  • 種屬：
    Pongo abelii (Sumatran orangutan)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
    蘇門達(dá)臘猩猩DBIRD復(fù)合物亞基KIAA1967同源物，也稱作ZC3H13，是DBIRD復(fù)合物的一個(gè)亞基，參與基因轉(zhuǎn)錄的調(diào)控。如果要重組該蛋白的部分，可以按照以下步驟進(jìn)行：
    克隆KIAA1967基因片段：可以使用PCR技術(shù)從蘇門達(dá)臘猩猩的基因組DNA中擴(kuò)增KIAA1967基因片段的序列，包括所需的編碼部分以及一些啟動(dòng)子和標(biāo)簽序列。
    構(gòu)建表達(dá)載體：將KIAA1967基因片段插入到表達(dá)載體中，可以選擇一些經(jīng)過驗(yàn)證的表達(dá)載體，如pET系列載體，這些載體中包含了一些促進(jìn)蛋白表達(dá)的元件，如啟動(dòng)子、增強(qiáng)子、終止子等。
    選擇表達(dá)宿主細(xì)胞：可以選擇一些常用的表達(dá)宿主細(xì)胞，如哺乳動(dòng)物細(xì)胞，例如CHO細(xì)胞、HEK293細(xì)胞等，并在細(xì)胞中轉(zhuǎn)染含有KIAA1967基因片段的表達(dá)載體。這些宿主細(xì)胞中還需要添加一些輔助蛋白質(zhì)，如轉(zhuǎn)染脂質(zhì)、抗生素等，以幫助蛋白質(zhì)的正確折疊和純化。
    蛋白表達(dá)和純化：在表達(dá)宿主細(xì)胞中誘導(dǎo)KIAA1967蛋白的表達(dá)，并通過細(xì)胞破碎和離心等方法提取蛋白質(zhì)片段。然后使用親和層析、離子交換層析、凝膠過濾層析等方法對(duì)蛋白進(jìn)行純化。
    蛋白質(zhì)的鑒定和活性檢測(cè)：使用SDS-PAGE、Western blot等方法對(duì)純化后的KIAA1967蛋白進(jìn)行鑒定和定量。此外，還可以使用細(xì)胞實(shí)驗(yàn)等方法對(duì)KIAA1967蛋白的生物活性進(jìn)行檢測(cè)，如檢測(cè)其在DBIRD復(fù)合物中的作用，以及其對(duì)基因轉(zhuǎn)錄的調(diào)控等。
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Mediates ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation. Represses the ligand-dependent transcriptional activation function of ESR2. Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1. Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization. Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway. Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3. Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2. Represses the transcriptional activator activity of BRCA1. Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro.
  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle.
  • 數(shù)據(jù)庫鏈接：