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  4. Recombinant Rat 3-alpha-hydroxysteroid dehydrogenase (Akr1c9)

Recombinant Rat 3-alpha-hydroxysteroid dehydrogenase (Akr1c9)

  • 中文名稱：
    Recombinant Rat 3-alpha-hydroxysteroid dehydrogenase(Akr1c9)
  • 貨號：
    CSB-YP027173RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rat 3-alpha-hydroxysteroid dehydrogenase(Akr1c9)
  • 貨號：
    CSB-EP027173RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rat 3-alpha-hydroxysteroid dehydrogenase(Akr1c9)
  • 貨號：
    CSB-EP027173RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rat 3-alpha-hydroxysteroid dehydrogenase(Akr1c9)
  • 貨號：
    CSB-BP027173RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rat 3-alpha-hydroxysteroid dehydrogenase(Akr1c9)
  • 貨號：
    CSB-MP027173RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Akr1c9
  • Uniprot No.：
  • 別名：
    Akr1c93-alpha-hydroxysteroid dehydrogenase; 3-alpha-HSD; EC 1.1.1.50; Hydroxyprostaglandin dehydrogenase
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-322
  • 氨基酸序列
    MDSISLRVAL NDGNFIPVLG FGTTVPEKVA KDEVIKATKI AIDNGFRHFD SAYLYEVEEE VGQAIRSKIE DGTVKREDIF YTSKLWSTFH RPELVRTCLE KTLKSTQLDY VDLYIIHFPM ALQPGDIFFP RDEHGKLLFE TVDICDTWEA MEKCKDAGLA KSIGVSNFNC RQLERILNKP GLKYKPVCNQ VECHLYLNQS KMLDYCKSKD IILVSYCTLG SSRDKTWVDQ KSPVLLDDPV LCAIAKKYKQ TPALVALRYQ LQRGVVPLIR SFNAKRIKEL TQVFEFQLAS EDMKALDGLN RNFRYNNAKY FDDHPNHPFT DE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Besides being a 3-alpha-hydroxysteroid dehydrogenase, the enzyme can accomplish diverse functions: as quinone reductase, as an aromatic alcohol dehydrogenase, as dihydrodiol dehydrogenase, and as 9-, 11-, and 15-hydroxyprostaglandin dehydrogenase.
  • 基因功能參考文獻：
    1. Ziram inhibited SRD5A1 and AKR1C14 activities, thus possibly interfering with neurosteroid production in rats. PMID: 28707553
    2. say stuff PMID: 28552457
    3. analysis of the complete kinetic mechanism rat liver 3alpha-HSD (AKR1C9) PMID: 17848571
    4. characterization of recombinant 3-alpha-hydroxysteroid dehydrogenase from liver: molecular weight; amino acid sequence; sequence alignment and homology with similar enzymes; inhibition by indomethacin PMID: 22217852
    5. Binding cavity residues of 3-alpha-hydroxysteroid dehydrogenase not only control steroid hormone affinity but also affect the rate of oxidation, which becomes rate-limiting when the steroid fails to make proper contact with these residues. PMID: 15134457
    6. 3 alpha-HSD immunoreactivity was largely distributed in the white matter and gray matter, but the highest density was found in sensory areas of the dorsal horn. PMID: 15305365
    7. Burst phase kinetics for the NADP(+)-dependent turnover of 3alpha-diol by W227A of 3Alpha-hydroxysteroid dehydrogenase was also abolished PMID: 15379543

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Aldo/keto reductase family
  • 組織特異性：
    In brain, highest levels found in olfactory bulb. Moderate levels present in cerebellum, cerebral cortex, hypothalamus and pituitary. Low levels present in amygdala, brain stem, caudate putamen, cingulate cortex, hippocampus, midbrain, and thalamus.
  • 數(shù)據(jù)庫鏈接：