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  4. Recombinant Rat 3-mercaptopyruvate sulfurtransferase (Mpst)

Recombinant Rat 3-mercaptopyruvate sulfurtransferase (Mpst)

  • 中文名稱：
    大鼠Mpst重組蛋白
  • 貨號：
    CSB-YP014770RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Mpst重組蛋白
  • 貨號：
    CSB-EP014770RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Mpst重組蛋白
  • 貨號：
    CSB-EP014770RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Mpst重組蛋白
  • 貨號：
    CSB-BP014770RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Mpst重組蛋白
  • 貨號：
    CSB-MP014770RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Mpst
  • Uniprot No.：
  • 別名：
    Mpst; 3-mercaptopyruvate sulfurtransferase; MST; EC 2.8.1.2
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    2-297
  • 氨基酸序列
    AAPQLFRAL VSAQWVAEAL KSPRASQPLK LLDASWYLPK LGRDARREFE ERHIPGAAFF DIDRCSDHTS PYDHMLPSAT HFADYAGSLG VSAATHVVIY DGSDQGLYSA PRVWWMFRAF GHHSVSLLDG GFRYWLSQNL PISSGKSPSE PAEFCAQLDP SFIKTHEDIL ENLDARRFQV VDARAAGRFQ GTQPEPRDGI EPGHIPGSVN IPFTEFLTSE GLEKSPEEIQ RLFQEKKVDL SKPLVATCGS GVTACHVVLG AFLCGKPDVP VYDGSWVEWY MRAQPEHVIS QGRGKTL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
  • 基因功能參考文獻：
    1. Presented is alternative pathway of production of hydrogen sulfide and/or polysulfides from (poly)sulfurated catalytic-site cysteine of reaction intermediates of MST via thioredoxin reduction. PMID: 29331374
    2. observations indicate that 3MST is probably not responsible for the increased production of H2S following permanent middle cerebral artery occlusion. PMID: 23805308
    3. 3-Mercaptopyruvate sulphurtransferase and not cystathionine gamma-lyase is the primary regulator of coronary artery hydrogen sulfide production and function. PMID: 26519030
    4. Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, cysteine aminotransferase (CAT) and mercaptopyruvate sulfurtransferase (MPST). PMID: 24611772
    5. 3MST exists in the neurons of medullary respiratory nuclei and its expression can be up-regulated by chronic intermittent hypoxia in adult rat PMID: 24051007
    6. SiRNA silencing of 3-MPST reduced basal bioenergetic parameters and prevented the stimulating effect of 3-MP on mitochondrial bioenergetics. PMID: 23104984
    7. Thus, sulfur oxides are suggested to release in the redox cycle of persulfide of mercaptopyruvate sulfurtransferase. PMID: 22149235
    8. 3-mercaptopyruvate sulfurtransferase (3MST) and cysteine aminotransferase (CAT) are localized to vascular endothelium in the thoracic aorta and produce hydrogen sulfide. PMID: 19605461
    9. the redox state regulates MST activity at the enzymatic level, and MST controls redox to maintain cellular redox homeostasis PMID: 16107337

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  • 亞細胞定位：
    Cytoplasm. Mitochondrion. Cell junction, synapse, synaptosome.
  • 組織特異性：
    Expressed in liver, heart, kidney and brain. Localizes to tubular epithelium in the kidney, pericentral hepatocytes in the liver, cardiac cells in the heart and neuroglial cells in the brain. Also expressed in vascular endothelium of the thoracic aorta. W
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