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  4. Recombinant Saccharomyces cerevisiae ATP-dependent helicase RRM3 (RRM3), partial

Recombinant Saccharomyces cerevisiae ATP-dependent helicase RRM3 (RRM3), partial

  • 中文名稱：
    釀酒酵母RRM3重組蛋白
  • 貨號：
    CSB-YP327886SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RRM3重組蛋白
  • 貨號：
    CSB-EP327886SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RRM3重組蛋白
  • 貨號：
    CSB-EP327886SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RRM3重組蛋白
  • 貨號：
    CSB-BP327886SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RRM3重組蛋白
  • 貨號：
    CSB-MP327886SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RRM3
  • Uniprot No.：
  • 別名：
    RRM3; RTT104; YHR031C; ATP-dependent DNA helicase RRM3; EC 3.6.4.12; Regulation of Ty1 transposition protein 104; rDNA recombination mutation protein 3
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    5' to 3' DNA replicative helicase recruited to paused replisomes to promote fork progression throughout nonhistone protein-DNA complexes, naturally occurring impediments that are encountered in each S phase where replication forks pauses. Needed for normal fork progression through over 1000 discrete sites scattered throughout the genome, like rDNA, tRNA genes, centromeres, active replication origins, or transcriptional silencers. Required for timely replication of the telomere and subtelomeric DNA and for wild-type levels of telomeric silencing. Involved in regulation of Ty1 transposition and protects the genome from instability at nascent sites of retrotransposition. Involved in DNA repair during stalled replication fork, regulation of fragile sites expression and essential for genome stability. Plays also a role in mtDNA replication. Has G-quadruplex (G4) unwinding activity and can suppress G4-induced genome instability when PIF1 levels are low.
  • 基因功能參考文獻：
    1. Rrm3 recruitment to replication-born DSBs is crucial for viability. PMID: 28475600
    2. RRM3 regulates epigenetic conversions in Saccharomyces cerevisiae in conjunction with Chromatin Assembly Factor I PMID: 27645054
    3. Rrm3 helicase facilitates replication through the transcription-dependent replication fork pause site and reduces recombination. PMID: 15775982
    4. Results show that deletion of the RRM3 gene significantly rescues the petite-induction phenotype of a Pif1p mutation and define a novel role for Rrm3p in mitochondrial function. PMID: 15907372
    5. Replication of all yeast chromosomes was markedly delayed in rrm3 cells. PMID: 17114583

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome, telomere.
  • 蛋白家族：
    Helicase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YHR031C

    STRING: 4932.YHR031C