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  4. Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase I subunit RPA43 (RPA43)

Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase I subunit RPA43 (RPA43)

  • 中文名稱：
    釀酒酵母RPA43重組蛋白
  • 貨號：
    CSB-YP025360SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RPA43重組蛋白
  • 貨號：
    CSB-EP025360SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RPA43重組蛋白
  • 貨號：
    CSB-EP025360SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RPA43重組蛋白
  • 貨號：
    CSB-BP025360SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RPA43重組蛋白
  • 貨號：
    CSB-MP025360SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RPA43
  • Uniprot No.：
  • 別名：
    RPA43; RRN12; YOR340C; O6271; DNA-directed RNA polymerase I subunit RPA43; A43; DNA-directed DNA-dependent RNA polymerase 36 kDa polypeptide
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-326
  • 氨基酸序列
    MSQVKRANEN RETARFIKKH KKQVTNPIDE KNGTSNCIVR VPIALYVSLA PMYLENPLQG VMKQHLNPLV MKYNNKVGGV VLGYEGLKIL DADPLSKEDT SEKLIKITPD TPFGFTWCHV NLYVWQPQVG DVLEGYIFIQ SASHIGLLIH DAFNASIKKN NIPVDWTFVH NDVEEDADVI NTDENNGNNN NEDNKDSNGG SNSLGKFSFG NRSLGHWVDS NGEPIDGKLR FTVRNVHTTG RVVSVDGTLI SDADEEGNGY NSSRSQAESL PIVSNKKIVF DDEVSIENKE SHKELDLPEV KEDNGSEIVY EENTSESNDG ESSDSD
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. Through its association with RRN3 is involved in recruitment of Pol I to rDNA promoters. In vitro, the A13-A43 subcomplex binds single-stranded RNA.
  • 基因功能參考文獻(xiàn)：
    1. Initiation-competent RNA polymerase I A43 subunit is associated with Rrn3, which stabilize A43. PMID: 27418187
    2. Spt6 physically associates with a Pol I subunit (Rpa43). PMID: 25918242
  • 亞細(xì)胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    Eukaryotic RPA43 RNA polymerase subunit family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YOR340C

    STRING: 4932.YOR340C