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  4. Recombinant Saccharomyces cerevisiae DNA replication ATP-dependent helicase DNA2 (DNA2), partial

Recombinant Saccharomyces cerevisiae DNA replication ATP-dependent helicase DNA2 (DNA2), partial

  • 中文名稱：
    釀酒酵母DNA2重組蛋白
  • 貨號：
    CSB-YP327901SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母DNA2重組蛋白
  • 貨號：
    CSB-EP327901SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母DNA2重組蛋白
  • 貨號：
    CSB-EP327901SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母DNA2重組蛋白
  • 貨號：
    CSB-BP327901SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母DNA2重組蛋白
  • 貨號：
    CSB-MP327901SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    DNA2; YHR164C; DNA replication ATP-dependent helicase/nuclease DNA2 [Includes: DNA replication nuclease DNA2; EC 3.1.-.-); DNA replication ATP-dependent helicase DNA2; EC 3.6.4.12)]
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is atypical: it cannot load onto its tracking strand internally and has an absolute free 5'-end requirement. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
  • 基因功能參考文獻：
    1. Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1. PMID: 27779184
    2. role of the Dna2 translocase activity in DNA break end resection and in the imposition of the 5' strand specificity of end resection. PMID: 28336516
    3. A model is proposed where Dna2 alone is responsible for cleaving of RPA-bound long DNA flaps. PMID: 26175049
    4. Data show that Dna2 is required for normal telomeric CA-strand fill-in. PMID: 23963457
    5. Nuclease activity of Saccharomyces cerevisiae Dna2 inhibits its potent DNA helicase activity. PMID: 23671118
    6. N-terminal 45-kDa domain of Dna2 endonuclease/helicase targets the enzyme to secondary structure DNA PMID: 23344960
    7. Saccharomyces cerevisiae Dna2, a nuclease-helicase that is essential for Okazaki fragment maturation, is employed specifically during S phase to stimulate Mec1 kinase and initiate the replication checkpoint PMID: 23355394
    8. Results show that the yeast Dna2 cysteine cluster is indeed an Fe-S domain and present biochemical and genetic evidence that this domain plays a role not only in nuclease but also in helicase activity. PMID: 22684504
    9. Cdk1-dependent phosphorylation of the resection nuclease Dna2 at Thr4, Ser17 and Ser237 stimulates its recruitment to double-strand breaks. PMID: 21841787
    10. The DNA repair functions of Dna2 contribute to its essential function, and in the absence of this repair function, the cell cycle inhibition by the checkpoint leads to permanent arrest. PMID: 21508669
    11. Dna2 exhibits a unique strand end-dependent helicase function PMID: 20929864
    12. Dna2p and then FEN1 track to remove initiator RNA via long flap intermediates during Okazaki fragment maturation PMID: 15448135
    13. DNA2 of Saccharomyces cerevisiae selectively unwinds triplet repeat DNA. PMID: 16085654
    14. report here two novel activities of both the yeast and human Dna2 helicase/nuclease protein: single strand annealing and ATP-independent strand exchange on short duplexes PMID: 17032657
    15. Replication protein A stimulates the 5'- to 3'-nuclease activity of Dna2 and inhibits the 3'- to 5'-exo/endonuclease activity of Dna2. PMID: 18593712
    16. Dna2 and replication protein A coordinate their functions for efficient flap cleavage and preparation for flap endonuclease 1 PMID: 18799459

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  • 亞細胞定位：
    Nucleus. Chromosome. Note=Recruited to double-strand. break (DSB) sites following phosphorylation at Ser-17 and Ser-237.
  • 蛋白家族：
    DNA2/NAM7 helicase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YHR164C

    STRING: 4932.YHR164C