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  4. Recombinant Saccharomyces cerevisiae EH domain-containing and endocytosis protein 1 (EDE1), partial

Recombinant Saccharomyces cerevisiae EH domain-containing and endocytosis protein 1 (EDE1), partial

  • 中文名稱：
    釀酒酵母EDE1重組蛋白
  • 貨號(hào)：
    CSB-YP335906SVG
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母EDE1重組蛋白
  • 貨號(hào)：
    CSB-EP335906SVG
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母EDE1重組蛋白
  • 貨號(hào)：
    CSB-EP335906SVG-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母EDE1重組蛋白
  • 貨號(hào)：
    CSB-BP335906SVG
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母EDE1重組蛋白
  • 貨號(hào)：
    CSB-MP335906SVG
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    EDE1
  • Uniprot No.：
  • 別名：
    EDE1; BUD15; YBL047C; YBL0501; YBL0520EH domain-containing and endocytosis protein 1; Bud site selection protein 15
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Functions at the internalization step of the clathrin-mediated endocytosis (CME) as an early-acting scaffold protein. Requires clathrin adapter proteins, ENT1/2 and YAP1801/2, for normal spatiotemporal dynamics and viability. Binds to biological membranes in a ubiquitin-dependent manner.
  • 基因功能參考文獻(xiàn)：
    1. When expressed in yeast, human Eps15 localized to the plasma membrane, where it recruited late-phase CME proteins and supported productive endocytosis, identifying it as an Ede1 functional homologue. PMID: 28057762
    2. Hrr25 kinase and Ede1 cooperate to initiate and stabilize endocytic sites PMID: 25625208
    3. a new and widely applicable tool to generate conditionally phosphorylated proteins to identify those whose dephosphorylation is required for cytokinesis. This approach identifies Aip1, Ede1 and Inn1 as cytokinetic regulators. PMID: 25371407
    4. Results show that oligomerization of Ede1 is essential for its localization and function as an early endocytic scaffold protein. PMID: 25366307
    5. Deubiquitination regulates formation of endocytic sites and stability of the endocytic coat and occurs through Ede1, because permanently ubiquitinated Ede1 phenocopies deletion of UBP2 and UBP7. PMID: 24746795
    6. Ede1 ubiquitin associated domain-ubiquitin interaction reveal several key affinity determinants and, unexpectedly, a negative affinity determinant in the wild-type Ede1 protein PMID: 16563434

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  • 亞細(xì)胞定位：
    Cytoplasm. Note=Localized to actin cortical patches concentrated in the developing bud tip in cells with small buds and at the mother-daughter neck in cells undergoing cytokinesis. Localization can be maintained in the absence of polymerized actin filaments.
  • 蛋白家族：
    VDP/USO1/EDE1 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YBL047C

    STRING: 4932.YBL047C