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  4. Recombinant Saccharomyces cerevisiae GU4 nucleic-binding protein 1 (ARC1)

Recombinant Saccharomyces cerevisiae GU4 nucleic-binding protein 1 (ARC1)

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae GU4 nucleic-binding protein 1(ARC1)
  • 貨號：
    CSB-YP342021SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae GU4 nucleic-binding protein 1(ARC1)
  • 貨號：
    CSB-EP342021SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae GU4 nucleic-binding protein 1(ARC1)
  • 貨號：
    CSB-EP342021SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae GU4 nucleic-binding protein 1(ARC1)
  • 貨號：
    CSB-BP342021SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae GU4 nucleic-binding protein 1(ARC1)
  • 貨號：
    CSB-MP342021SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ARC1
  • Uniprot No.：
  • 別名：
    ARC1; G4P1; YGL105W; G3085; tRNA-aminoacylation cofactor ARC1; Acyl-RNA-complex protein 1; GU4 nucleic-binding protein 1; G4p1 protein; P42; tRNA-interacting factor ARC1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-376
  • 氨基酸序列
    MSDLVTKFES LIISKYPVSF TKEQSAQAAQ WESVLKSGQI QPHLDQLNLV LRDNTFIVST LYPTSTDVHV FEVALPLIKD LVASSKDVKS TYTTYRHILR WIDYMQNLLE VSSTDKLEIN HDLDLPHEVI EKKKKAPAGG AADAAAKADE DVSKKAKKQD HPRGKPDEET LKKLREEAKA KKAAKKAANA KQQQEQQNKA PEKPKPSAID FRVGFIQKAI KHPDADSLYV STIDVGDEEG PRTVCSGLVK HFPLDAMQER YVVVVCNLKP VNMRGIKSTA MVLCGSNDDK VEFVEPPKDS KAGDKVFFEG FGDEAPMKQL NPKKKIWEHL QPHFTTNDGL EVIFKDEEEK DHPVRKLTNA KGESFKVASI ANAQVR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Binds to tRNA and functions as a cofactor for the methionyl-tRNA synthetase (MetRS) and glutamyl-tRNA synthetase (GluRS). Forms a complex with MetRS and GluRS and increases their affinity for cognate tRNAs due to the presence of a tRNA binding domain in its middle and C-terminal part. Binds specifically G4 quadruplex nucleic acid structures (these are four-stranded right-handed helices, stabilized by guanine base quartets). Also required for cytoplasmic confinement of the synthetases and tRNA.
  • 基因功能參考文獻：
    1. study suggests that the structure and function of Arc1p can be modulated via biotinylation in response to temperature changes. PMID: 27330079
    2. An ab initio model of both trimeric and the pentameric complexes containing Arc1,GluRS and MetRS. PMID: 23161686
    3. Present study suggests a more complex picture for Arc1p-mediated stimulation of yeast tRNA glutamylation than just a role in increasing the glutamyl-tRNA synthetase binding affinity for tRNAGlu. PMID: 15667228
    4. cytoplasmic location of Arc1p is maintained by Xpo1p-dependent nuclear export PMID: 15710377
    5. dual localization of (c)ERS is controlled by binding to Arc1p PMID: 19417106

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  • 亞細胞定位：
    Cytoplasm. Note=Largely excluded from the nucleus.
  • 蛋白家族：
    TRNA-aminoacylation cofactor ARC1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YGL105W

    STRING: 4932.YGL105W