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  4. Recombinant Saccharomyces cerevisiae Mediator of replication checkpoint protein 1 (MRC1), partial

Recombinant Saccharomyces cerevisiae Mediator of replication checkpoint protein 1 (MRC1), partial

  • 中文名稱：
    釀酒酵母MRC1重組蛋白
  • 貨號：
    CSB-YP338680SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MRC1重組蛋白
  • 貨號：
    CSB-EP338680SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MRC1重組蛋白
  • 貨號：
    CSB-EP338680SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MRC1重組蛋白
  • 貨號：
    CSB-BP338680SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MRC1重組蛋白
  • 貨號：
    CSB-MP338680SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    MRC1; YCL061C; YCL61C/YCL60C; Mediator of replication checkpoint protein 1; DNA replication checkpoint mediator MRC1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

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靶點詳情

  • 功能：
    Required for normal DNA replication. Phosphorylated in response to DNA replication stress. Phosphorylation allows it to mediate the activation of RAD53.
  • 基因功能參考文獻：
    1. An unbiased kinome screening identified several kinases that phosphorylate Mrc1 at the N terminus upon different environmental stresses. PMID: 29371596
    2. Dia2-dependent degradation of Mrc1 is required for replication fork restart in a pathway that includes Sgs1 and Mph1. PMID: 27956499
    3. Disruption of MRC1 leads to consistently elevated expansion and contraction rates of CAG.CTG repeat tracts. PMID: 18321795
    4. Authors show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1. PMID: 23165348
    5. Whereas Ddc1 and Dpb11 aid in replication-checkpoint activation, colocalization of Mec1 and Mrc1 at stalled replication forks promotes Rad53 activation sufficient to stabilize the replisome during transient replication stress. PMID: 22298423
    6. Data show that that ISC1 controls cell morphology in combination with checkpoint regulators Swe1, Cdk1(CDC28), MRC1, TOF1, CSM3, RAD9 and Rad53. PMID: 21840863
    7. cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-type firing times despite over twofold lengthening of S phase PMID: 20219942
    8. Data show that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and when the replication fork is stalled. PMID: 15755447
    9. Mrc1 functions constitutively to promote normal replication fork progression. PMID: 16137624
    10. Tof1p-Csm3p function differently from MRC1 and their complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae PMID: 16418273
    11. Mrc1 plays a specific role in DNA replication, promoting the Srs2 recruitment to proliferating cell nuclear antigen (PCNA) independently of checkpoint signaling. PMID: 16724109
    12. Mrc1 may negatively regulate Cdc45 and MCM helicase to render stalled forks capable of resuming replication. PMID: 16849602
    13. Study reports that in the absence of Mrc1, a component of the replication forks, telomeres of cdc13 or yku70 mutants exhibited increased degradation, while telomerase-negative cells displayed accelerated senescence. PMID: 17323081
    14. analyzed sister-chromatid cohesion in double mutants of mrc1Delta, tof1Delta, and csm3Delta and identified additive cohesion defects that indicated the existence of at least two pathways that contribute to sister-chromatid cohesion PMID: 17483413
    15. Mrc1 and Tof1 control the normal progression of DNA replication forks in distinct ways. PMID: 17652453
    16. In unperturbed cells, Mrc1 interacts independently with both the N-terminal and C-terminal halves of Pol2 (Pol2N and Pol2C). PMID: 18851837
    17. Data show that the replication checkpoint suppresses the formation of Rad52 foci in an Mrc1-dependent manner and prevents homologous recombination (HR) at chromosome breaks induced by the HO endonuclease. PMID: 19322196
    18. Rad53 is activated by Mec1 through adaptor protein Mrc1 PMID: 19457865
    19. Mec1-dependent, Rad53-independent phosphorylation of Mrc1 is required to establish a positive feedback loop that stabilizes Mec1 and the replisome at stalled forks. PMID: 19515819
    20. An Mcm helicase acts as a checkpoint sensor for methyl methanesulfonate-induced DNA damage through direct binding to the replication checkpoint mediator Mrc1. PMID: 19620285
    21. Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks PMID: 19819872

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  • 亞細胞定位：
    Nucleus. Note=Associated with chromatin during S phase.
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YCL061C

    STRING: 4932.YCL061C